The second edition of Experiments in Plant Tissue Culture makes available new information that has resulted from recent advances in the applications of plant tissue culture techniques to agriculture and industry. This comprehensive laboratory text takes the reader through a graded series of experimental protocols and also provides an introductory review of each topic. Topics include: a plant tissue culture laboratory, aseptic techniques, nutritional components of media, callus induction, organ formation, xylem cell differentiation, root cultures, cell suspensions, micropropagation, embryogenesis, isolation and fusion of protoplasts, haploid cultures, storage of plant genetic resources, secondary metabolite production, and quantification of procedures. This volume offers all of the basic experimental methods for the major research areas of plant tissue culture, and it will be invaluable to undergraduates and research investigators in the plant sciences.
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The new edition of a guide to the essentials of plant tissue culture has been completely revised and updated. The organization has been improved, and new illustrations as well as experiments on such topics as potato callus formation and embryo culture have been added.
"This well thought out book does considerably more than is claimed in the title. It not only takes the reader through a graded series of experimental protocols covering the major techniques used in plant tissue culture, it also provides a good introductory review of each topic." Trends in Biochemical Sciences
"...a worthy text and an excellent addition to any plant science laboratory." British Society for Developmental Biology
"This small book is a gem....The revisions have kept the same, excellent format, but have added, updated and refined information and experiments and rearranged topics to form a more logical sequence....The result is an excellent volume that fulfills its promises and carries on the success of the earlier versions: It is an excellent primer. The new arrangement is fine....I especially appreciated the reasoned analysis of past discoveries, the effort to discuss the basic biology of responses in cultures, and the attention to the small details that often mean the difference between success and failure....This compact, informative little jewel of a book should serve that audience, and many others, very well indeed." Philip V. Ammirato, New Biological Books
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