During the last three decades the theoretical and practical approaches of those studying animal viruses have been profoundly influenced by two events. Of these the first was the development of new techniques and the refinement and extension of older procedures which permit the recognition and measurement of the pathogenic effects of viruses “in vitro” and “in vivo”. The application of these methods soon led to the isolation from man and other warm blooded vertebrates of hundreds of agents whose existence had been wholly unsuspected or only vaguely postulated, as well as to the cultivation of agents already well known and which had proved difficult or impossible to propagate in the laboratory. Somewhat later, the concept of a science of molecular biology was formulated. By applying the principles and procedures of this discipline to problems in their own field virologists have succeeded within the short space of last ten years in defining with increasing precision the nature, the properties, and the mode of replication of many of these agents.
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