Recombinant DNA Principles and Methodologies

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9780824799892: Recombinant DNA Principles and Methodologies

This comprehensive yet balanced work emphasizes the principles and rationale underlying recombinant DNA methodology while furnishing a general understanding of the experimental protocols-suggesting flexible approaches to resolving particular molecular necessities that are easily adaptable to readers' specific applications.
Features summary tables presenting at-a-glance information on practices of recombinant DNA methodologies!

Recombinant DNA Principles and Methodologies discusses basic and advanced topics requisite to the employment of recombinant DNA technology, such as

  • plasmid biology
  • nucleic acid biochemistry
  • restriction enzymes
  • cloning strategies
  • gel electrophoresis
  • southern and northern blotting
  • preparation of probes
  • phage lambda biology
  • cosmids and genome analysis
  • cloned gene expression
  • polymerase chain reaction
  • conventional and automated DNA sequencing
  • site-directed mutagenesis
  • and more!
    Elucidating the material with over 2250 edifying references, equations, drawings, and photographs, this state-of-the-art resource is a valuable hands-on guide for molecular and cell biologists, biochemists, bioprocess technologists, applied and industrial microbiologists, virologists, geneticists, chemical engineers, and upper-level undergraduate and graduate students in these disciplines.
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    Review:


    ...provide[s] clear insights into the biological, chemical, and physical rationale behind common molecular methods used in lab manuals and commericial kits.
    - Trends in Genetics

    "About this title" may belong to another edition of this title.

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