Interpreting Protein Mass Spectra: A Comprehensive Resource - Hardcover

Snyder, A. Peter

Hardcover

ISBN 10: 0841235716 ISBN 13: 9780841235717

Publisher: American Chemical Society, 2000

Synopsis

Interpreting Protein Mass Spectra is a hands-on laboratory guide for a wide range of researchers investigating the structures of proteins and peptides. The focus is on the interpretation of structural information gathered through electrospray ionization-mass spectrometry (ESI-MS). The book will also provide useful background and protocols for anyone using matrix-assisted laser desorption/ionization (MALDI), fast atom bombardment (FAB), and secondary ion mass spectrometry (SIMS). The book includes numerous practical examples, detailed discussions of experimental setups, and valuable hints for troubleshooting both methods and the handling of materials.

About this title

About the Author

Peter Snyder is at U.S. Army Edgewood Chemical Biological Center.

Review

"Mass spectrometry rather than protein mass is the concern of Snyder, a research chemist for the British Army. He explains his chosen methods for interpreting the primary structure of proteins as well as their post-translation modifications. He presents electrospray ionization- mass spectrometry (ESI-MS) as one of the more attractive tools for determining protein integrity and structure, but also outlines the use of high pressure liquid chromatography, capillary electrophoresis, and tandem mass spectrometry. Highlighting phosphoproteins and glycoproteins, he shows how to analyze separate peaks and details within a high mass peak, which give the purity, reproducibility, and signal optimization characteristics of the mass spectrum of a protein. He intends his treatment to be a course text for beginning graduate students and a reference for researchers. It was developed in partnership with the American Chemical Society."--SciTech Book News

"Those wishing to understand and, perhaps, interpret such macromolecular data will be well rewarded by using this as a study guide." -- Doody's

Edition details

• Publisher: American Chemical Society
• Publication date: 2000
• ISBN 10: 0841235716
• ISBN 13: 9780841235717
• Binding: Hardcover
• Language: English
• Edition number: 1
• Number of pages: 544