Gene Transfer: Delivery and Expression of DNA and RNA, A Laboratory Manual - Hardcover

Hardcover

ISBN 10: 0879697644 ISBN 13: 9780879697648

Publisher: Cold Spring Harbor Laboratory Press, 2006

Synopsis

Understanding gene function and regulation requires rigorous testing in live cells and organisms. Recent advances have provided a variety of new strategies for delivering DNA and RNA into cells and probing their expression, as well as new clinical applications that rely upon the introduction of genetic material. The vast number of available techniques for clinical and laboratory research often makes selecting the optimal method a difficult process. Gene Transfer: Delivery and Expression of DNA and RNA provides the first comprehensive guide to technical approaches for delivering nucleic acids into cells and organisms and of ensuring (even manipulating) appropriate expression. The detailed, step-by-step protocols cover a variety of methods, both well established and newly evolving. These include viral and nonviral methods of gene delivery, transgenic approaches, strategies for the regulation of transgene expression, and modification of the host response. The introductory matter to each chapter includes concise technical and theoretical discussions with considerations for selection of the appropriate system and strategies for delivery.

About this title

About the Author

Theodore Friedmann is affiliated with the University of California, San Diego. John Rossi is affiliated with the Beckman Research Institute of the City of Hope, Duarte, California.

Edition details

• Publisher: Cold Spring Harbor Laboratory Press
• Publication date: 2006
• Language: English
• ISBN 10: 0879697644
• ISBN 13: 9780879697648
• Binding: Hardcover
• Edition number: 1
• Number of pages: 793
• Editor: Friedmann Theodore, Rossi John