Imaging in Developmental Biology: A Laboratory Manual - Hardcover

Hardcover

ISBN 10: 0879699396 ISBN 13: 9780879699390

Publisher: Cold Spring Harbor Laboratory Press, 2010

Synopsis

New imaging technologies have revolutionized the study of developmental biology. Where researchers once struggled to connect events at static timepoints, imaging tools now offer the ability to visualize the dynamic form and function of molecules, cells, tissues, and whole embryos throughout the entire developmental process. Imaging in Developmental Biology: A Laboratory Manual, a new volume in Cold Spring Harbor Laboratory Press' Imaging series, presents a comprehensive set of essential visualization methods. The manual features primers on live imaging of a variety of standard model organisms including C. elegans, Drosophila, zebrafish, Xenopus, avian species, and mouse. Further techniques are organized by the level of visualization they provide, from cells to tissues and organs to whole embryos. Methods range from the basics of labeling cells to cutting-edge protocols for high-speed imaging, optical projection tomography, and digital scanned laser light-sheet fluorescence. Imaging has become a required methodology for developmental biologists, and Imaging in Developmental Biology: A Laboratory Manual provides the detailed explanations and instructions for mastering these necessary techniques.

About this title

About the Author:

James Sharpe is Head of Outstation and Senior Scientist at EMBL-CRG Systems Biology Unit, Barcelona, Spain.

Review:

Imaging in Developmental Biology: A Laboratory Manual is another outstanding and practical manual in the Cold Spring Harbor Laboratory Press s Imaging series. I highly recommend this book as the textbook for a laboratory course at the undergraduate and graduate level. This is a laboratory manual and it will complement, but not replace, a full course in developmental biology....I posit that without a laboratory course on imaging in developmental biology, or extensive practical work under a skilled mentor s supervision, it is unlikely that the researcher will succeed...Imaging in Developmental Biology provides an excellent practical solution to this dilemma....My perusal of the entire book permits me to state that the chapters are well written, the protocols are clear and written with sufficient detail, and the illustrations and images are extremely helpful to the reader. --Biomedical Optics Express

Imaging in Developmental Biology is an excellent resource from which both novices and experienced researchers can obtain current state-of-the-art embryo-imaging protocols for studying key developmental events, such as cell-fate determination, morphogen gradient formation, cell-cell interactions, cell migration and morphogenesis....comment often expressed was: I did not know that we could do so much! Upon first perusal, this comprehensive book seems almost overwhelming with an impressive 57 chapters and seven appendices. But it does contain just about everything known about imaging embryos. This is not surprising as the volume is based, in part, on the popular and excellent Cold Spring Harbor imaging course....The book is pleasant to read, with its clever use of illustrations, photos and online protocol videos....we highly recommend this book and hope that multidisciplinary collaborative expertise in biology, imaging, image analysis, computer science, visualization and database construction will continue to fast-forward 4D imaging techniques and, consequently, our knowledge of development. --Development

About this edition

• Publisher: Cold Spring Harbor Laboratory Press
• Publication date: 2010
• ISBN 10: 0879699396
• ISBN 13: 9780879699390
• Binding: Hardcover
• Edition number: 1
• Number of pages: 883
• Editor: Sharpe James, Wong Rachel, Yuste Rafael