PCR Protocols: Current Methods and Applications (Methods in Molecular Biology) - Softcover

Softcover

ISBN 10: 0896032442 ISBN 13: 9780896032446

Publisher: Humana Press, 1993

Synopsis

The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book's focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols.

Key Topics and Techniques: basic startup procedures for PCR • PCR in detecting DNA and RNA • PCR in DNA synthesis and mutagenesis • PCR cloning of DNA • selection of primers • radioactive and nonradioactive labeling • quantitation of PCR products by HPLC • chromosome assignment • PCR mapping of HLA genes • sequencing of PCR products • genomic footprinting • quantitation of tumor gene expression • screening of phage libraries • SSP-PCR and genome walking • subcloning of PCR products.

About this title

Review

...affords many new insights and contributions of the first order...an excellent book of the highest interest.-Cellular and Molecular Biology

Edition details

• Publisher: Humana Press
• Publication date: 1993
• Language: English
• ISBN 10: 0896032442
• ISBN 13: 9780896032446
• Binding: Paperback
• Edition number: 1
• Number of pages: 392
• Editor: White Bruce A.