Drug'DNA Interaction Protocols (Methods in Molecular Biology)

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9780896034471: Drug'DNA Interaction Protocols (Methods in Molecular Biology)
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A collection of useful molecular techniques to illuminate and explore the interaction of drugs and ligands with DNA. These easily reproducible methods involve sequence recognition properties, as well as the physical approaches for measuring both the strength of interaction and the mode of drug binding to DNA. The interactions are also examined from a cellular perspective and for their usefulness in the design of new therapeutic agents. The powerful techniques detailed here will be particularly useful in elucidating the action of existing therapeutic agents, as well as in the design of new anti-cancer drugs with improved action and reduced toxicity.

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The last few years have witnessed the creation of new generations of sequence reading compounds, which have incredible potential for targeting specific DNA sequences. In Drug-DNA Interaction Protocols, Second Edition, expert researchers explore these compounds, revealing how a deeper understanding of the structure of DNA, along with an improved ability to manipulate it, has led to much progress in recent years. Chapters describe a new arsenal of techniques for probing the interactions between drugs and DNA, including various biophysical techniques for quantifying these interactions and for describing them in molecular and atomic detail, and they comprehensively consider both molecular and cellular approaches. Composed in the highly successful Methods in Molecular Biology™ series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls.

 Authoritative and current, Drug-DNA Interaction Protocols, Second Edition, is a critical volume that includes a wide range of chapters, which together provide methods for assessing the strength and mode of binding, the sequence selectivity, and the effect on biological systems.

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"Methods in Molecular Biology is a long standing, respected series that provides protocols for the researcher. ..this particular book is a welcome addition for the researcher. It is recommended for all medical and science libraries."-Reviewer: H. Robert Malinowsky, E-STREAMS

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