Mycobacteria Protocols (Methods in Molecular Biology) - Hardcover

Neil G. Stoker Tanya Parish

Hardcover

ISBN 10: 0896034712 ISBN 13: 9780896034716

Publisher: Humana Press, 2010

Synopsis

Laboratory experts present cutting-edge molecular biology techniques for studying the mycobacteria, a medically significant group of organisms responsible for both human and animal disease, including tuberculosis and leprosy. The methods range from basic culture techniques, cell fractionation, and nucleic acid purification to more advanced protocols for the introduction of cloned DNA into mycobacteria, the use of reporter genes, and the expression of foreign genes. There are also important techniques for mycobacteriophages, genome analysis, chemical and transposon mutagenesis, gene replacement, and analysis of gene expression, as well as such clinically useful techniques as antibiotic susceptibility testing, speciation, and RFLP analysis.

About this title

From the Back Cover

Due to the rising threat of tuberculosis and other mycobacterial infections, methods to study the biology of the mycobacteria and to improve diagnostic, therapeutic and preventative reagents are still very much in need.  Mycobacteria Protocols, Second Edition updates and refines the methods of the well-received first edition while adding newly developed methods culled from the most cutting-edge research in the field.  Ranging from the basics of sub-cellular fractionation to advanced methods using specialized growth conditions and whole genome, transcriptome and proteome analysis, the chapters conform to the Methods in Molecular Biology™ series format, providing step-by-step laboratory protocols, lists of necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Mycobacteria Protocols, Second Edition is the perfect resource to promote and stimulate further research into these intriguing, important and most fractious of bacteria.

Review

"...this book is an essential purchase for an laboratory dealing with mycobacteria and good value for individual researchers."
-Microbiology Today

"...a unique book...presents excellent description of many of the molecular biological tools that have been developed...an invaluable collection of methodologies for mycobacterial research...the molecular mycobacteriologist will find much of interest in this book...Worth getting." -Journal of Medical Microbiology

"...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms...of interest to the epidemiologist is the description of computer analysis of DNA fingerprinting for tuberculosis while the chapter on accessing MycDB, a database dedicated to mycobacterial research, will be of value to all those working in this area."-Molecular Biotechnology

"...this book provides a thorough guide to many of the techniques currently used to study the Mycobacteria. Comprehensive treatment of the subject includes both molecular techniques and the basic microbiological methods required for manipulation of these organisms....The detailed technical nature of each chapter provides clear instruction for those wishing to engage in this area of research." - Molecular Biotechnology

Edition details

• Publisher: Humana Press
• Publication date: 2010
• Language: English
• ISBN 10: 0896034712
• ISBN 13: 9780896034716
• Binding: Hardcover
• Edition number: 1
• Number of pages: 488