This book provides extensive protocols on the use of PCR for molecular cloning, including the cloning of unknown sequences, cloning of unknown sequences, and cloning of gene family members. It also covers the use of PCR for various approaches to mutagenesis and recombination, its use for differential display and subtractive approaches, and its use for generating and screening gene libraries and sublibraries.
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This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis. - Biochemical Education
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