Dendritic Cell Protocols (Methods in Molecular Biology) - Hardcover

Andrew J. Stagg

Hardcover

ISBN 10: 0896035840 ISBN 13: 9780896035843

Publisher: Humana Press, 2001

Synopsis

Stephen P Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.

About this title

From the Back Cover

It is now apparent that dendritic cells not only play important roles in the body's immune system through their complex interactions with T cells, B cells, and other cell types, but also possess distinct functional attributes that enable them to assume different roles in that system. In Dendritic Cell Protocols, Stephen P. Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these so-called "veiled" cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide variety of tissues or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. Variant methods that have been successful in other laboratories have been included to expand experimental possibilities. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeutics.

State-of-the-art and highly practical, Dendritic Cell Protocols provides both experienced and novice investigators with powerful tools to illuminate the complex biology of these foundation cells-cells that shape the evolution of acquired immune responses, play important roles in innate immunity, and promise the development of powerful new immunotherapeutics.

Review

". . .the purposes of the book are worthwhile and are well attained. . .The book could be used effectively by experienced or beginning researchers in the field since the protocols are written clearly and concisely. There are 38 brief chapters in this book by over 70 authors, who are among the most experienced and knowledgeable researchers in the field. . .Among the many experimental protocols described in this book are methods for isolating, culturing, and establishing cell lines from many organs from murine and human sources. The protocols are written clearly and should serve as a useful laboratory resource for anyone wishing to learn how to study these cells involved in the first line of defense of the immune system. The book has a utilitarian look and feel. . .It would be quite useful for anyone doing bench research on murine or human dendritic cells."-Doody's Health Sciences Book Review Journal "The layout of the protocols is in a step-by-step fashion which makes them relatively easy to follow. Moreover, they contain enough of the fine details that are so often disregarded in published scientific manuscripts, thus ensuring their reproducibility. Hence, Dendritic Cell Protocols will prove to be an extremely useful source of practical information for novice DC researchers." - Immunology News

Edition details

• Publisher: Humana Press
• Publication date: 2001
• ISBN 10: 0896035840
• ISBN 13: 9780896035843
• Binding: Hardcover
• Language: English
• Edition number: 1