Celiac Disease: Methods and Protocols (Methods in Molecular Medicine, 41) - Hardcover

Hardcover

ISBN 10: 0896036502 ISBN 13: 9780896036505

Publisher: Humana, 2000

Synopsis

Research into gluten sensitivity has never been more popular nor more exciting. Thus a call for a new book, Celiac Disease: Methods and Protocols, devoted entirely to techniques and technology seemed a most appropriate undertaking. I am therefore grateful to Professor J. M. Walker for inviting me to complete this task for Humana Press. To do this would have been imp- sible without the contributions of friends and colleagues from around the world who have devoted so much interest to the project. It has also been necessary for them to master the unique chapter-writing skills required of every ma- script published in this series of laboratory monographs. With regard to gluten sensitivity we are in a period of great change, occasioned by the introduction of reproducible methods for cloning lymp- cytes, the application of physical methods to identify gluten sequences as T-cell antigens, the study of peptide responses in vitro and in vivo by either jejunal or rectal challenge, elucidating the locations of other genes concerned in pathogenesis, or the use of elegant immunohistocytochemical and mRNA probing techniques for analyzing the finer points of the mucosal inflam- tory response to gluten.

About this title

From the Back Cover:

With the recent development of powerful bioanalytical techniques, research on gluten sensitivity has entered an exciting new phase. In Celiac Disease: Methods and Protocols, Professor Michael N. Marsh, a recognized world authority on this condition, together with a team of other expert laboratory/clinical investigators from around the world present a collection of these state-of-the-art techniques for studying the biology and immunopathology of celiac disease. Both novice and experienced researcher will find in this collection detailed step-by-step methods for cloning lymphocytes, creating gene/peptide libraries, and performing genotyping, linkage, and positional cloning. Also included are techniques for determining the peptide structure of HLA-bound material (tandem mass spectroscopy), computerized morphometry, in situ hybridization, organ culture, and monoclonal AB assays.
Comprehensive and cutting-edge, Celiac Disease: Methods and Protocols provides all experimental and clinical investigators with the essential core of readily reproducible methods necessary for successful work on celiac disease today.

About the Author:

Anthony W. Ryan PhD Room 1.17, Institute of Molecular Medicine Trinity Centre for Health Sciences Trinity College Dublin St James's Hospital, Dublin 8, Ireland. tel +353 1 896 3273 (lab 896 3292) fax +353 1 454 2043 e-mail aryan12@tcd.ie

About this edition

• Publisher: Humana
• Publication date: 2000
• ISBN 10: 0896036502
• ISBN 13: 9780896036505
• Binding: Hardcover
• Number of pages: 304
• Editor: Marsh Michael N.