Proteoglycan Protocols (Methods in Molecular Biology, 171) - Hardcover

Hardcover

ISBN 10: 0896037592 ISBN 13: 9780896037595

Publisher: Humana, 2001

Synopsis

Proteoglycans are some of the most elaborate macromolecules of mammalian and lower organisms. The covalent attachment of at least five types of glycosami- glycan side chains to more than forty individual protein cores makes these molecules quite complex and endows them with a multitude of biological functions. Proteoglycan Protocols offers a comprehensive and up-to-date collection of prepa- tive and analytical methods for the in-depth analysis of proteoglycans. Featuring st- by-step detailed protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, to generate strategies for prokaryotic and eukaryotic expression, to utilize methods for the suppression of specific proteoglycan gene expression and for the detection of mutant cells and degradation products, and to study specific interactions between proteoglycans and extracellular matrix proteins as well as growth factors and their receptors. The readers will find concise, yet comprehensive techniques carefully drafted by leading experts in the field. Each chapter commences with a general Introduction, followed by a detailed Materials section, and an easy-to-follow Methods section. An asset of each chapter is the extensive notation that includes troubleshooting tips and practical considerations that are often lacking in formal methodology papers. The reader will find this section most valuable because it is clearly provided by experienced scientists who have first-hand knowledge of the techniques they outline. In addition, most of the chapters are well illustrated with examples of typical data generated with each method.

About this title

From the Back Cover

Proteoglycans, highly complex macromolecules found in mammalian and lower organisms, serve a multitude of biological functions. In Proteoglycan Protocols, Renato Iozzo, MD, and an authoritative team of investigators present for the first time a comprehensive and up-to-date collection of readily reproducible preparative and analytical methods for the in-depth analysis of these important compounds. Featuring step-by-step protocols, this book will enable both novice and experienced researchers to isolate intact proteoglycans from tissues and cultured cells, to establish the composition of their carbohydrate moieties, and to generate strategies for prokaryotic and eukaryotic expression. There are also detailed techniques for the suppression of specific proteoglycan genes, for the detection of mutant cells and their degradation products, and for studying specific interactions between proteoglycans and extracellular matrix proteins and with growth factors and their receptors. Each method is carefully described by leading experts in the field and includes practical considerations that are often lacking in formal papers.

Authoritative and detailed, Proteoglycan Protocols offers molecular and cellular biologists, biochemists, pathologists, and geneticists a comprehensive reference and practical guide to the most common methods used today for the isolation and characterization of proteoglycans from cultured cells, tissues, and biological fluids.

Edition details

• Publisher: Humana
• Publication date: 2001
• Language: English
• ISBN 10: 0896037592
• ISBN 13: 9780896037595
• Binding: Hardcover
• Number of pages: 574
• Editor: Iozzo Renato V.