Cytoskeleton Methods and Protocols (Methods in Molecular Biology) - Hardcover

Ray H. Gavin

Hardcover

ISBN 10: 0896037711 ISBN 13: 9780896037717

Publisher: Humana Press, 2001

Synopsis

Ray H. Gavin brings together an international panel of experienced researchers to detail the readily reproducible methods that utilize biochemistry, immunology, genetics, microscopy, and image analysis for investigating cytoskeleton structure and function. Each protocol contains proven step-by-step instructions that enable both the novice and the experienced researcher to achieve successful experimental results. The protocols utilize diverse model systems in a variety of organisms, including Saccharomyces, Micrasterias, Tetrahymena, Drosophila, Spisula, and Xenopus. Microscopy applications include digital-video microscopy and computer-assisted systems for the evaluation of cell motility and morphology. State-of-the-art and highly practical, Cytoskeleton Methods and Protocols makes available a diverse collection of powerful experimental systems and tools for successfully studying cytoskeleton structure and function.

About this title

From the Back Cover

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology™ series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.

Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.

Review

"The authors of the chapters are highly experienced and widely recognized as experts in their fields...The volume is one that the libraries of many universities and research institutes will want to own as a resource for their cytoskeleton researchers." - TRENDS in Cell Biology "The material and procedures sections of the chapters are generally well detailed such that one with little previous experience might be able to carry out the procedure or adapt it to one's own particular system. The chapters include helpful hints on the techniques which are often not found in published articles. As a microscopist, I was impressed by the simple but elegant procedure of placing a finger print on the corner of a cover slip in order to determine the plane of focus in video-enhanced microscopy, presented by George Langford in Chapter 4. It is often just those very kinds of things that can mean the difference between success and failure, or at least help to reduce the frustration level of a first timer." - Microscopy and Microanalysis "As a cell biologist, I was impressed by the chapters dealing with the study of cytoskeletal dynamics in living cells using green fluorescent protein and the study of organelle transport using chromatophores as tools you got the true impression you can do it thanks to the step-by-step protocols, you feel you cannot be wrong and I personally worked out one clear experiment. I will do on my own on mouse oocytes. I think the whole scientific community has to thank Dr. Gavin for providing us with such a helpful book." - European Journal of Histochemistry "Cytoskeleton: Methods and Protocols emphasizes an integrative approach and is intended for novice and advanced researchers. It is highly recommended for libraries serving researchers interested in cytoskeleton structure and function and related areas of cell biology. " - E-STREAMS

Edition details

• Publisher: Humana Press
• Publication date: 2001
• ISBN 10: 0896037711
• ISBN 13: 9780896037717
• Binding: Hardcover
• Language: English
• Edition number: 1
• Number of pages: 302