Rt-Pcr Protocols (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896038750 ISBN 13: 9780896038752

Publisher: Humana Pr Inc, 2002

Synopsis

A panel of highly regarded molecular biologists and clinical researchers describe in detail their most novel, useful, and interesting RT-PCR applications. Here the newcomer will find readily reproducible protocols for highly sensitive detection and quantification of gene expression, the in situ localization of gene expression in tissue, and the cloning of genes, as well as for analyzing T-cell clones and the differential expression of genes. For the expert seeking to extend the usefulness of RT-PCR, there are user-friendly applications that complement the latest technological advances, including laser-capture microdissection (LCM), real-time and quantitative PCR, microarray technology, cDNA cloning, and antibody engineering.

About this title

From the Back Cover

Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

About the Author

Joe O'Connell holds a Ph.D. in biochemistry and is a Senior Lecturer in the Department of Medicine at University College Cork, Ireland. Editor of RT-PCR Protocols (2002), he has contributed numerous articles and reviews to scientific journals. O'Connell was awarded a Centennial Prize for Academic Publishing in Medical and Health Sciences by the National University of Ireland in 2009. A native of Portmagee, County Kerry, O'Connell resides with his wife and son in Cork City. Dingle Day is his first published collection of poetry.

Edition details

• Publisher: Humana Pr Inc
• Publication date: 2002
• Language: English
• ISBN 10: 0896038750
• ISBN 13: 9780896038752
• Binding: Hardcover
• Edition number: 1
• Number of pages: 378
• Editor: O'Connell Joe