RT-PCR Protocols (Methods in Molecular Biology) - Hardcover

Joe O'Connell

Hardcover

ISBN 10: 0896038750 ISBN 13: 9780896038752

Publisher: Humana Press, 2010

Synopsis

In view of the ubiquity of the use of standard RT-PCR, the main objective of this volume is to provide novel, useful applications of RT-PCR. These include some useful adaptions and application that are relevant to the wider research communities who are already familiar with the basic RT-PCR protocol. For example, a variety of different adaptions are described that have been employed to obtain quantitative data from RT-PCR. Quantitative RT-PCR provides the ability to accurately measure changes/imbalances in specific mRNA expression between normal and diseased tissues. RT-PCR has afforded many opportunities in diagnostics, allowing sensitive detection of RNA viruses such as HIV and HCV. RT-PCR facilities many diverse techniques in research, including in situ localization of mRNA, antibody engineering, and cDNA cloning. In particular, this volume highlights how RT-PCR, microarray technology, HPLC and time-resolved fluorimetry. This volume will stimulate the reader towards diverse new ways in which this remarkable technique can facilitate the molecular aspects of their biomedical research.

About this title

From the Back Cover

Once a tedious, highly skilled operation, reverse-transcription polymerase chain reaction (RT-PCR) has become a routine and invaluable technique used in most laboratories. In RT-PCR Protocols, Second Edition, expert researchers fully update the technologies presented in the popular previous edition, such as competitive RT-PCR, nested RT-PCR, RT-PCR from single cells, and RT-PCR for cloning. In addition, newer technologies are also explored, including multiplex RT-PCR, RT-LATE-PCR, and the greatly advanced field of real-time quantitative RT-PCR, while recent advances in creating the optimum RT-PCR reaction, e.g. RNA extraction, primer design, and reverse transcription, end the book with their indispensable input. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.

User friendly and up-to-date, RT-PCR Protocols, Second Edition acts as a handy companion to scientists from numerous diverse backgrounds who wish to explore further the marvels of gene expression.

About the Author

Joe O'Connell holds a Ph.D. in biochemistry and is a Senior Lecturer in the Department of Medicine at University College Cork, Ireland. Editor of RT-PCR Protocols (2002), he has contributed numerous articles and reviews to scientific journals. O'Connell was awarded a Centennial Prize for Academic Publishing in Medical and Health Sciences by the National University of Ireland in 2009. A native of Portmagee, County Kerry, O'Connell resides with his wife and son in Cork City. Dingle Day is his first published collection of poetry.

Edition details

• Publisher: Humana Press
• Publication date: 2010
• ISBN 10: 0896038750
• ISBN 13: 9780896038752
• Binding: Hardcover
• Language: English
• Edition number: 1
• Number of pages: 380