Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology)

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9780896039728: Mitochondrial DNA: Methods and Protocols (Methods in Molecular Biology)

Internationally recognized authorities describe in great detail the methods they have perfected to analyze mtDNA and the proteins involved in its maintenance. The analytical techniques cover the purification of mtDNA from a variety of sources and the analysis of DNA for both deletions, point mutations, and damage, for replication intermediates, and for following the fate of mtDNA outside of the mitochondria. Additional analytical methods are presented for analyzing the proteins and enzymes that maintain mtDNA. Comprehensive and timely, Mitochondrial DNA: Methods and Protocols offers both basic and clinical researchers proven cutting-edge methods for analyzing mtDNA and illuminating the role it plays in the aging process, apoptosis, and possibly some cancers.

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"Accordingly, the book is timely and of significant use to investigators working in this area. The book is divided into two major sections -- the first deals with analysis of the DNA and the second with analysis of related proteins. A valuable introductory chapter discusses available animal models for mitochondrial disease and the book concludes with a discussion of knockout mice. Given the general interest in mitochondrial mutations as they relate to a variety of human diseases, this contribution is timely and of value. The protocols are sufficiently detailed to allow adaptation into any working laboratory."-Doody's Health Sciences Book Review Journal

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