MAP Kinase Signaling Protocols (Methods in Molecular Biology) - Hardcover

Hardcover

ISBN 10: 0896039986 ISBN 13: 9780896039988

Publisher: Humana Press, 2004

Synopsis

An outstanding collection of both classic and cutting-edge techniques for the detection and measurement of mitogen-activated protein kinase (MAPK) signaling cascades. The protocols includes methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the down regulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. The techniques offer biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed in to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

About this title

From the Back Cover

Mitogen-activated protein kinase (MAPK) signaling cascades initiate highly significant cellular processes and are involved in a large number of diseases, including cancer and diabetes. In MAP Kinase Signaling Protocols, leading researchers with extensive hands-on experience describe in detail both classic and cutting-edge techniques for the detection and measurement of the MAPKs and other components in these cascades. The protocols include methods for the determination of the subcellular localization of these components, the structural and biophysical analysis of the components, and identification of novel components of known and unknown signaling cascades. Additional methods examine the upstream mechanisms of activation of MAPK cascades by various receptors, the mechanisms involved in the downregulation of the MAPK cascades, and identification of targets of the MAPK cascades. A number of techniques use inhibitors in research on MAPK and transgenic mice for studies of MAPK signaling. Each readily reproducible protocol is described in step-by-step detail and includes a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Comprehensive and versatile, MAP Kinase Signaling Protocols offers biochemists, cell biologists, physicians, and biotechnologists all the essential laboratory techniques needed to conduct productive studies of MAPK signaling in health and disease, in measuring the influence of drugs, and in a broad range of experimental systems.

Review

...this book continues the strong tradition of the series by recruiting the most prominent investigators in the field to provide a detailed discussion of specialized topics with a focus on direct practical applications.-Doody's Health Sciences Book Review Journal

Edition details

• Publisher: Humana Press
• Publication date: 2004
• Language: English
• ISBN 10: 0896039986
• ISBN 13: 9780896039988
• Binding: Hardcover
• Edition number: 1
• Number of pages: 332
• Editor: Seger Rony