This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
"synopsis" may belong to another edition of this title.
This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.
"About this title" may belong to another edition of this title.
Seller: GreatBookPrices, Columbia, MD, U.S.A.
Condition: New. Seller Inventory # 47343494-n
Seller: Ria Christie Collections, Uxbridge, United Kingdom
Condition: New. In. Seller Inventory # ria9781071637012_new
Quantity: Over 20 available
Seller: GreatBookPrices, Columbia, MD, U.S.A.
Condition: As New. Unread book in perfect condition. Seller Inventory # 47343494
Seller: GreatBookPricesUK, Woodford Green, United Kingdom
Condition: New. Seller Inventory # 47343494-n
Quantity: Over 20 available
Seller: moluna, Greven, Germany
Gebunden. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge techniquesProvides step-by-step detail essential for reproducible resultsContains key implementation advice from the expertsThis detailed volume examines classical and cutting-edge methods involving double-stra. Seller Inventory # 1241470111
Quantity: Over 20 available
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This detailed volume examines classical and cutting-edge methods involving double-stranded RNA (dsRNA), specifically regarding isolation, visualization, characterization, production, and application. Many protocols, such as co-immunoprecipitation-based isolation of double-stranded RNA-associated protein complexes, identification of mycoviruses by dsRNA extraction, application of dsRNA for fungi disease management (Sclerotinia sclerotiorum and Botrytis cin), and production of double-stranded RNA in plants by plant viral vectors for gene silencing, can also be easily adapted for identification of viruses from other organisms, control of other pathogens, and fundamental research. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls.Reliable and practical, Double-Stranded RNA: Methods and Protocols serves as an ideal reference book for students and researchers who work with dsRNA. 144 pp. Englisch. Seller Inventory # 9781071637012
Quantity: 2 available
Seller: GreatBookPricesUK, Woodford Green, United Kingdom
Condition: As New. Unread book in perfect condition. Seller Inventory # 47343494
Quantity: Over 20 available
Seller: preigu, Osnabrück, Germany
Buch. Condition: Neu. Double-Stranded RNA | Methods and Protocols | Xiaofei Cheng (u. a.) | Buch | xi | Englisch | 2024 | Humana | EAN 9781071637012 | Verantwortliche Person für die EU: Springer Verlag GmbH, Tiergartenstr. 17, 69121 Heidelberg, juergen[dot]hartmann[at]springer[dot]com | Anbieter: preigu Print on Demand. Seller Inventory # 128029032
Quantity: 5 available
Seller: Kennys Bookshop and Art Galleries Ltd., Galway, GY, Ireland
Condition: New. 2024. Hardcover. . . . . . Seller Inventory # V9781071637012
Quantity: 15 available
Seller: buchversandmimpf2000, Emtmannsberg, BAYE, Germany
Buch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -Isolation of Double-Stranded RNAs by Lithium Chloride Fractionation.- Detection of dsRNA by Acridine Orange Staining.- Isolation of dsRNA from Plants by Cellulose Chromatography.- Rapid Purification of dsRNA Using Micro-Spin Cellulose Column.- Analysis of Virus-Induced Double-Stranded RNA in Living Plant Cells by the dRBFC Assay.- Detection of dsRNA with the Fluorescence In Situ Hybridization (FISH) Assay.- Subcellular Colocalization Assay of Host Factors with Viral Replication Complex in the dsRNA Reporter Nicotiana benthamiana.- Production of Double-Stranded RNA Using the Prokaryotic Promoter-Mediated Bidirectional Transcription.- Inducible Expression of dsRNA in Escherichia coli.- In Vivo Production of dsRNA Using Bacteriophage ¿6 in Pseudomonas syringae Cit7 Cells.- Bacteria-Based Double-Stranded RNA Production to Develop Cost-Effective RNA Interference Application for Insect Pest Management.- Transiently Induce RNA Silencing in Plants Using a TobaccoNecrosis Virus A (TNV-A)-Based dsRNA Production System.- Co-Immunoprecipitation-Based Isolation of Double-Stranded RNA-Associated Protein Complexes in Nicotiana benthamiana.- Analysis of Plant Virus-Induced Immunity by Using Viral-Derived Double-Stranded RNA in Arabidopsis thaliana.- Identification of Mycoviruses by dsRNA Extraction.- Production of Double-Stranded RNA In Planta by a Potato Mop-Top Virus (PMTV)-Based Vector for Inducing Gene Silencing.- Application of dsRNA for Fungi Disease Management Sclerotinia sclerotiorum and Botrytis cinerea.- Application of dsRNA in the Pine Wood Nematode, Bursaphelenchus xylophilus.Libri GmbH, Europaallee 1, 36244 Bad Hersfeld 156 pp. Englisch. Seller Inventory # 9781071637012
Quantity: 1 available