Adipose-Derived Stem Cells: Methods and Protocols (Methods in Molecular Biology, 2783) - Softcover

Softcover

ISBN 10: 1071637649 ISBN 13: 9781071637647

Publisher: Humana, 2025

Synopsis

Since the publication of the previous editions, there has been increased focus on the use of adipose-derived stromal/stem cells (ASC) and stromal vascular fraction (SVF) cells in three-dimensional hydrogel-based scaffolds for the development of microphysiological systems (MPS) serving as in vitro humanized assays and alternatives to in vivo pre-clinical animal models. This third edition volume discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Adipose-Derived Stem Cells: Methods and Protocols, Third Edition is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field.  

About this title

From the Back Cover

Since the publication of the previous editions, there has been increased focus on the use of adipose-derived stromal/stem cells (ASC) and stromal vascular fraction (SVF) cells in three-dimensional hydrogel-based scaffolds for the development of microphysiological systems (MPS) serving as in vitro humanized assays and alternatives to in vivo pre-clinical animal models. This third edition volume discusses of the latest technology and advancements in the field of human-derived ASC and SVF. The chapters in this book are organized into four parts. Part One focuses on human ASC’s isolation, characterization, and differentiation. Part Two describes the isolation and characterization of ASC and SVF from canine, feline, and murine tissues. Part Three looks at hydrogels, scaffolds, and microphysiological systems, and Part Four talks about the new assays and applications using ASC. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and comprehensive, Adipose-Derived Stem Cells: Methods and Protocols, Third Edition is a valuable resource for both novice and experts researchers interested in learning more about this important and developing field.  

Edition details

• Publisher: Humana
• Publication date: 2025
• Language: English
• ISBN 10: 1071637649
• ISBN 13: 9781071637647
• Binding: Paperback
• Edition number: 3
• Number of pages: 408
• Editor: Gimble Jeffrey, Bunnell Bruce, Frazier Trivia, Sanchez Cecilia