Current Protocols Select: Methods and Applications in Microscopy and Imaging

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9781118044315: Current Protocols Select: Methods and Applications in Microscopy and Imaging
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Compiled by editors with hands-on experience in microscopy, teaching, and protocol design and communication, this book provides a practical, bench-side guide to the various methods and applications of the advanced light microscope in the cell biology laboratory. It offers detailed step-by-step instructions written at a level that lets investigators employ even very sophisticated microscopy methods. The result is a resource for seasoned investigators and those new to the use of the microscope alike.

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From the Back Cover:

The advanced light microscope is a highly sophisticated robotic tool which, when coupled with appropriate detectors and image processing, allows scientists to investigate the inner workings of biological systems from the single molecule, through cells, to the entire living organism. The last twenty-five years have brought significant growth in the utility of the microscope, especially given novel imaging tools (e.g., the computerized microscope and the confocal microscope), reagents (fluorescent proteins), and powerful computers that can process enormous quantities of data produced by these technologies. Today, the application of the light microscope is no longer a limited descriptive field but a battery of extremely complex, rich, and useful primary research tools. It is evident that the successful integration of these technologies is fundamental to bench biomedical research.

This book is intended to be a resource for those new to the use of the microscope as well as for seasoned investigators, exposing them to the wealth of opportunities to visualize the molecules and cellular processes they are studying. Because these are Current Protocols articles, they provide detailed step-by-step instructions for the various methods and applications, described and written at a level that lets every investigator employ even very sophisticated microscopy methods.

  • For graduate students, postdocs, and individuals who are branching into new areas as an entry into the specialized techniques of microscopy
  • A resource that encourages the use of the microscope beyond that of the simple light microscope
  • An essential reference for core facilities at academic institutions
  • An "instructional" text essential for each of these core resources

About the Author:

Simon C. Watkins, Ph.D.: Dr. Watkins is a Professor and Vice Chair of the Cell Biology and Physiology Department at the University of Pittsburgh, Pennsylvania, and holds a secondary appointment as Professor in the Department of Immunology at the same institution. He is also the Founder and Director of the Center for Biologic Imaging at the University of Pittsburgh. Dr. Watkins is an expert in microscopy and imaging and is an Editor for Current Protocols in Cytometry where he is in charge of the chapters on 'Image Cytometry Instrumentation' and 'Cellular and Molecular Imaging'.

Claudette M. St. Croix, Ph.D.: Dr. St. Croix is an Assistant Professor in the Department of Environmental and Occupational Health at the University of Pittsburgh, Pennsylvania, and holds a secondary appointment as Assistant Professor in the Cell Biology and Physiology Department at the same institution. She is also Assistant Director of the Center for Biologic Imaging at the University of Pittsburgh. Dr. St Croix's highly inventive applications of advanced imaging technologies have led to numerous invitations to present her work nationally and internationally and to take lead roles in well respected courses such as Quantitative Fluorescence Microscopy (run at Mount Desert Island Biology Laboratory) and at the national Microscopy Society of America annual meeting. She is also an author for Current Protocols in Cytometry.

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