Reversible Ligand Binding: Theory and Experiment - Hardcover

Bellelli, Andrea; Carey, Jannette

Hardcover

ISBN 10: 111923848X ISBN 13: 9781119238485

Publisher: Wiley, 2018

Synopsis

Presents the physical background of ligand binding and instructs on how experiments should be designed and analyzed

Reversible Ligand Binding: Theory and Experiment discusses the physical background of protein-ligand interactions—providing a comprehensive view of the various biochemical considerations that govern reversible, as well as irreversible, ligand binding. Special consideration is devoted to enzymology, a field usually treated separately from ligand binding, but actually governed by identical thermodynamic relationships. Attention is given to the design of the experiment, which aids in showing clear evidence of biochemical features that may otherwise escape notice. Classical experiments are reviewed in order to further highlight the importance of the design of the experiment. Overall, the book supplies students with the understanding that is necessary for interpreting ligand binding experiments, formulating plausible reaction schemes, and analyzing the data according to the chosen model(s).

Topics covered include: theory of ligand binding to monomeric proteins; practical considerations and commonly encountered problems; oligomeric proteins with multiple binding sites; ligand binding kinetics; hemoglobin and its ligands; single-substrate enzymes and their inhibitors; two-substrate enzymes and their inhibitors; and rapid kinetic methods for studying enzyme reactions.

• Bridges theory of ligand binding and allostery with experiments
• Applies historical and physical insight to provide a clear understanding of ligand binding
• Written by a renowned author with long-standing research and teaching expertise in the area of ligand binding and allostery
• Based on FEBS Advanced Course lectures on the topic

Reversible Ligand Binding: Theory and Experiment is an ideal text reference for students and scientists involved in biophysical chemistry, physical biochemistry, biophysics, molecular biology, protein engineering, drug design, pharmacology, physiology, biotechnology, and bioengineering.

About this title

From the Back Cover

Presents the physical background of ligand binding and instructions on designing and analyzing experiments

Reversible Ligand Binding: Theory and Experiment discusses the physical background of protein-ligand interactions—providing a comprehensive view of the principles that govern reversible, as well as irreversible, ligand binding. Special consideration is devoted to enzymology, a field usually treated separately from ligand binding, but actually governed by identical thermodynamic relationships. Attention is given to the design of experiments, including how to uncover evidence of biochemical features that may otherwise escape notice. Classical experiments are reviewed in order to further highlight the importance of the experimental design. Overall, the book supplies students with understanding necessary for interpreting ligand binding experiments, formulating plausible reaction schemes, and analyzing the data according to a chosen model.

Topics covered include: theory of ligand binding to monomeric proteins; practical considerations and commonly encountered problems; oligomeric proteins with multiple binding sites; ligand binding kinetics; hemoglobin and its ligands; single-substrate enzymes and their inhibitors; two-substrate enzymes and their inhibitors; and rapid kinetic methods for studying enzyme reactions.

• Bridges theory and experiment in ligand binding and allostery
• Applies historical and physical insight to provide clear understanding of ligand binding
• Written by renowned authors with long research and teaching expertise in the areas of ligand binding and allostery

Reversible Ligand Binding: Theory and Experiment is an ideal text for students and scientists involved in biophysical chemistry, physical biochemistry, biophysics, molecular biology, protein engineering, drug design, pharmacology, physiology, biotechnology, or bioengineering.

About the Author

Andrea Bellelli, PhD is a Professor of Biochemistry at the University of Rome Sapienza. He chaired the Department of Biochemical Sciences "A. Rossi Fanelli" and currently chairs the Medicine and Surgery "B" school at the same University. His research focuses on structural and functional properties of oxygen carrying proteins.

Jannette Carey, PhD is a Professor of Chemistry at Princeton University and a visiting scientist of the Academy of Sciences of the Czech Republic at Nové Hrady, where she initiated and organizes a biennial FEBS practical and lecture course, Ligand binding theory and practice.

About this edition

• Publisher: Wiley
• Publication date: 2018
• ISBN 10: 111923848X
• ISBN 13: 9781119238485
• Binding: Hardcover
• Edition number: 1
• Number of pages: 304