Items related to RNA Nanotechnology and Therapeutics: Methods and Protocols
Synopsis
This volume contains a compilation of techniques and laboratory protocols directly related to RNA nano technology and its applications in nano biotechnology and nano medicine. The chapters in this book cover a wide range of research methods that can be easily comprehended and carried out in a step-by-step manner by graduate students and postdoctoral fellows from diverse scientific disciplines. Some of the covered topics include various biochemical, biophysical and bioinformatics approaches for analyzing RNA structures and properties; methods to analyze the multi-step assembly process of RNA nano architectures; purification of multifunctional RNA nanoparticles by ultracentrifugation and HPLC; real-time detection of RNA nano constructs in vivo; conjugation of imaging, targeting and therapeutic modules to RNA scaffold; and design and characterization of RNA-protein nanostructures. Written in the highly successful Methods of Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and key tips on troubles troubleshooting and avoiding known pitfalls. Cutting-edge and practical, RNA Nanotechnology and Therapeutics: Methods and Protocols propels the field toward new heights by engaging researchers working in the diverse fields of physical, engineering, and life sciences.
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About the Author
Peixuan Guo, Ph. D. William Farish Endowed Chair in Nano biotechnology Professor of School of Pharmacy Director of Nano biotechnology Center University of Kentucky Director of NCI Cancer Nanotechnology Platform Partnership Program: RNA Nanotechnology for Cancer Therapy Mailing address College of Pharmacy University of Kentucky 565 Biopharm Complex 789 S. Limestone Street Lexington, KY 40536 Phone: (Office) (859)218-0128 (Cell Phone): (513)728-1411 peixuan.guo@uky.edu (future)
Review
"The main strength of this book is in its focus on RNA as a functional agent. RNA can be derivatized using a variety of biochemical technologies to incorporate therapeutic or imaging functionalities. This book spans different aspects of techniques that are necessary to obtain functional RNA nanoparticles, including computational design, synthesis of components, assembly of functional units, and their biochemical and biophysical characterization. It also discusses promises and challenges in the applications of RNA nanoparticles as therapeutics. ... This book is unique in that it covers a very broad spectrum of topics related to RNA nanotechnology, yet it maintains a focus on constructing and analyzing RNA nanoparticles. It introduces the reader to the basics of RNA structure, and discusses functions of various natural RNAs, from small molecules to large molecular machines such as the ribosome. It also introduces the concept of designing new RNAs, and provides a comprehensive coverage of modern techniques, linking theory and experiment. Technologies discussed include RNA structure prediction, prediction of interactions, simulations of RNA folding, computational design, chemical methods for RNA nanoparticle synthesis, assembly, and labeling, as well as methods for their biophysical and biochemical characterization and biomolecular imaging. ... Chapters 1, 4, 7 are very exciting. Chapter 1 is a condensed introduction to the topic of RNA nanotechnology and to challenges and opportunities in using the RNA molecules as therapeutic agents. It is quite obvious that Dr. Guo is an enthusiast of RNA nanotechnology and in fact, this enthusiasm is quite contagious. Chapters 4 and 7 provide more focused description of two issues: 1) naturally existing RNAs as molecular machines, with a very informative discussion of differences between molecular machines and macro-machines, using ribosome as an example and 2) the role of dynamics (as opposed to 3D structure alone) in RNA design. Both these chapters demonstrate the extraordinary interdisciplinary character of the field of RNA nanotechnology, as well asof this very book. I definitely want to have this book on my shelf, and I intend to read all chapters as soon as I get it!"
––Prof. Janusz M. Bujnicki, International Institute of Molecular and Cell Biology in Warsaw, and Adam Mickiewicz University in Poznań, Poland
"This book is very good in this field. I want to have this book on my shelf. ... This book has a broad coverage from principles and fundamentals of RNA nanotechnology, RNA nanotechnology design and construction to diagnostic and therapeutic application. These detailing descriptions provide a clear and complete picture of up-to-date knowledge of RNA nanotechnology and applications."
––Prof. Shu-Feng Zhou, University of South Florida
"The range of topics is excellent. ... A nice collection of, basic and focused, articles on RNA."nanotechnology, a fast developing field."
―Eric Westhof, IBMC-CNRS, Université de Strasbourg, France
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