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Synthetic mRNA: Production, Introduction Into Cells, and Physiological Consequences (Methods in Molecular Biology, 1428) - Hardcover
Synopsis
This volume
presents detailed laboratory protocols for in
vitro synthesis of mRNA with favorable properties, its introduction into
cells by a variety of techniques, and the measurement of physiological and
clinical consequences such as protein replacement and cancer immunotherapy. Synthetic
techniques are described for structural features in mRNA that provide investigational
tools such as fluorescence emission, click chemistry, photo-chemical
crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced
activation of the innate immune response. Protocols are described for clinical
applications such as large-scale transfection of dendritic cells, production of
GMP-grade mRNA, redirecting T cell specificity, and use of molecular
adjuvants for RNA vaccines. Written
in the highly successful Methods in
Molecular Biology series format, chapters include introductions to their
respective topics, lists of the necessarymaterials and reagents, step-by-step
laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Synthetic
mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource
for both laboratory investigators and clinicians interested in this powerful
and rapidly evolving technology.
"synopsis" may belong to another edition of this title.
From the Back Cover
This volume presents detailed laboratory protocols for in vitro synthesis of mRNA with favorable properties, its introduction into cells by a variety of techniques, and the measurement of physiological and clinical consequences such as protein replacement and cancer immunotherapy. Synthetic techniques are described for structural features in mRNA that provide investigational tools such as fluorescence emission, click chemistry, photo-chemical crosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reduced activation of the innate immune response. Protocols are described for clinical applications such as large-scale transfection of dendritic cells, production of GMP-grade mRNA, redirecting T cell specificity, and use of molecular adjuvants for RNA vaccines. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessarymaterials and reagents, step-by-step laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resource for both laboratory investigators and clinicians interested in this powerful and rapidly evolving technology.
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Gebunden. Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Includes cutting-edge methods and protocolsProvides step-by-step detail essential for reproducible resultsContains key notes and implementation advice from the expertsIncludes cutting-edge methods and protocolsProvides ste. Seller Inventory # 107269846
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology. 332 pp. Englisch. Seller Inventory # 9781493936236
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Buch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology. 332 pp. Englisch. Seller Inventory # 9781493936236
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Buch. Condition: Neu. Neuware -This volumepresents detailed laboratory protocols for invitro synthesis of mRNA with favorable properties, its introduction intocells by a variety of techniques, and the measurement of physiological andclinical consequences such as protein replacement and cancer immunotherapy. Synthetictechniques are described for structural features in mRNA that provide investigationaltools such as fluorescence emission, click chemistry, photo-chemicalcrosslinking, and that produce mRNA with increased stability in the cell, increased translational efficiency, and reducedactivation of the innate immune response. Protocols are described for clinicalapplications such as large-scale transfection of dendritic cells, production ofGMP-grade mRNA, redirecting T cell specificity, and use of molecularadjuvants for RNA vaccines. Writtenin the highly successful Methods inMolecular Biology series format, chapters include introductions to theirrespective topics, lists of the necessarymaterials and reagents, step-by-steplaboratory protocols, and tips on troubleshooting and avoiding known pitfalls.SyntheticmRNA: Production, Introduction into Cells, and Physiological Consequences is a valuable and cutting-edge resourcefor both laboratory investigators and clinicians interested in this powerfuland rapidly evolving technology. Seller Inventory # 9781493936236
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