Items related to CRISPR Gene Editing: Methods and Protocols

CRISPR Gene Editing: Methods and Protocols - Hardcover

 
9781493991693: CRISPR Gene Editing: Methods and Protocols
Hardcover
ISBN 10: 1493991698 ISBN 13: 9781493991693
Publisher: Humana, 2019

Synopsis

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPR gene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

"synopsis" may belong to another edition of this title.

From the Back Cover

This detailed volume guides readers through strategic planning and user-friendly guidelines in order to select the most suitable CRISPR-Cas system and target sites with high activity and specificity. Methods covering CRISPR gRNA design, CRISPR delivery, CRISPR activity quantification (indel quantification), and examples of applying CRISPR gene editing in human pluripotent stem cells, primary cells, gene therapy, and genetic screening are included. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 


Authoritative and invaluable, CRISPR Gene Editing: Methods and Protocols will assist undergraduates, graduates, and researchers with detailed guidelines and methods for the vitally important CRISPRgene editing field. 

Chapter 3 is available open access under a CC BY 4.0 license via link.springer.com.

"About this title" may belong to another edition of this title.

Publisher
Humana
Publication date
2019
Language
English
ISBN 10 
1493991698
ISBN 13 
9781493991693
Binding
Hardcover
Edition number
1
Number of pages
373
Editor
Luo Yonglun