Pediatric Hematology: Methods and Protocols (Methods in Molecular Medicine, 91) - Hardcover

Hardcover

ISBN 10: 1588290433 ISBN 13: 9781588290434

Publisher: Humana, 2003

Synopsis

Much of the progress in the diagnosis, classification, and treatment of childhood hematological disorders has come from a partnership between clinicians and scientists. Indeed, access to molecular techniques is now an integral part of the practice of modern pediatric hematology. The aim of Pediatric Hematology: Methods and Protocols is to provide a collection of scientific protocols that cover the major aspects of the discipline. Most clinicians will be familiar with the difficulties inherent in establishing the underlying diagnosis in genetic marrow failure syndromes. A particular concern is failure to diagnose those associated with DNA repair defects. In Chapter 1, Dokal and colleagues present simple protocols for the molecular investigation of Fanconi anemia and dyskeratosis congenita. Molecular diagnosis is also important in children with congenital pure red cell aplasia, owing to the highly variable phenotype of this condition. In Chapter 2, Ball describes relevant protocols for the investigation of Diamond–Blackfan anemia. Hereditary hemoglobinopathy is a major cause of death and morbidity throughout the world. This area has seen great advances in screening and antenatal diagnosis. In Chapter 3, Old details protocols for the molecular diagnosis of most forms of hemoglobinopathy. High-quality, accurate molecular testing on small amounts of material has been fundamental to progress in antenatal diagnostics. The introduction to his comprehensive chapter includes a discussion of the general principles that underpin these studies. In contrast to hemoglobinopathy, severe hemophilia is uncommon.

About this title

From the Back Cover

Molecular techniques are now an integral part of the practice of modern pediatric hematology. In Pediatric Hematology: Methods and Protocols, hands-on masters of laboratory techniques describe their best methods for investigating and detecting a wide variety of hematological disorders. Here, the reader will find cutting-edge molecular protocols for the diagnosis of Fanconi anemia, dyskeratosis congenita, immunodeficiency, and most forms of hemoglobinopathy. In addition, there are detailed methods for molecular human platelet antigen genotyping, an effective PCR procedure for thrombophilia screening, and protocols for fluorescent in situ hybridization for cases in which insufficient metaphases are recovered or cryptic translocations are not visible. Since the measurement of minimal residual disease (MRD) provides a much more accurate risk-directed therapy, three methods are presented for detecting residual leukemia below the threshold of light microscopy, along with relatively simple, rapid, and inexpensive methods for the detection of MRD in ALL and AML. On the cutting-edge of technology, microarrays are used for the analysis of gene expression in childhood leukemia, and novel methods of graft engineering and reduced intensity conditioning regimens are applied to tissue typing for the assessment of donor/recipient chimerism. Each readily reproducible protocol is described in step-by-step detail and includes an introduction to the principle behind the method, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and highly practical, Pediatric Hematology: Methods and Protocols offers experimental and clinical investigators a gold-standard collection of reliable molecular techniques essential to the diagnosis, classification, and treatment of childhood hematological disorders.

Review

"Pediatric Hematology, Methods and Protocols, is an excellent reference book for physicians and scientists who serve pediatric patients in the specialty of hematology. This book is a valuable technical manual for laboratory scientists to set up new diagnostic tests and to troubleshoot and improve their current diagnostic methods. It is also a very practical guideline for physicians to choose relevant tests to arrive at correct diagnoses in light of clinical history and symptoms. The book is well organized, concisely written, and easy to understand. To the best of my knowledge, no other book describes the molecular diagnostic methods of pediatric hematologic disorders in such detail. I highly recommend this book for laboratories of hematology and molecular diagnosis in hospitals serving pediatric patients." -Society for Pediatric Pathology

"The information in this book is clearly expressed...a useful and practical text for laboratory scientists..." - Journal of Pediatrics and Child Health

About this edition

• Publisher: Humana
• Publication date: 2003
• ISBN 10: 1588290433
• ISBN 13: 9781588290434
• Binding: Hardcover
• Number of pages: 284
• Editor: Goulden Nicholas J., Steward Colin G.