A comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Common downstream applications such as mutagenesis of plasmids, recombinant protein expression, and the use of reporter genes, are also described.
"synopsis" may belong to another edition of this title.
Manipulation of recombinant DNA, which is almost exclusively performed using the host E. coli, constitutes one of the fundamental methodologies of molecular biotechnology. In E. coli Plasmid Vectors, experienced bench researchers describe their proven techniques for the manipulation of recombinant plasmids utilizing this popular bacterial host. The authors describe readily reproducible methods for cloning DNA into plasmid vectors, transforming plasmids into E. coli, and analyzing recombinant clones. They also include protocols for the construction and screening of libraries, as well as specific techniques for specialized cloning vehicles, such as cosmids, bacterial artificial chromosomes, l vectors, and phagemids. Also presented are methods for common downstream applications, such as mutagenesis, expression of recombinant proteins and RNA transcripts, and uses of reporter genes. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, tips on troubleshooting and avoiding known pitfalls and, where needed, a discussion of the interpretation and use of the results.
Comprehensive and highly practical, E. coli Plasmid Vectors offers those new to the field a basic guide to the use of plasmid vectors in the cloning host E. coli, and those more experienced researchers a broad-ranging, proven array of successful techniques.
"About this title" may belong to another edition of this title.
US$ 33.54 shipping from United Kingdom to U.S.A.
Destination, rates & speedsSeller: Books Puddle, New York, NY, U.S.A.
Condition: New. pp. 332. Seller Inventory # 26251229
Quantity: 1 available
Seller: Majestic Books, Hounslow, United Kingdom
Condition: New. pp. 332 Illus. Seller Inventory # 7629442
Quantity: 1 available
Seller: Biblios, Frankfurt am main, HESSE, Germany
Condition: New. pp. 332. Seller Inventory # 18251223
Quantity: 1 available
Seller: Basi6 International, Irving, TX, U.S.A.
Condition: Brand New. New. US edition. Expediting shipping for all USA and Europe orders excluding PO Box. Excellent Customer Service. Seller Inventory # ABEJUNE24-193325
Quantity: 1 available
Seller: BennettBooksLtd, North Las Vegas, NV, U.S.A.
hardcover. Condition: New. In shrink wrap. Looks like an interesting title! Seller Inventory # Q-1588291510
Quantity: 1 available
Seller: Mispah books, Redhill, SURRE, United Kingdom
Hardcover. Condition: Like New. Like NewLIKE NEW. book. Seller Inventory # ERICA83415882915103
Quantity: 1 available
Seller: Basi6 International, Irving, TX, U.S.A.
Condition: Brand New. New. US edition. Expediting shipping for all USA and Europe orders excluding PO Box. Excellent Customer Service. Seller Inventory # ABEJUNE24-193326
Quantity: 1 available
Seller: Lucky's Textbooks, Dallas, TX, U.S.A.
Condition: New. Seller Inventory # ABLIING23Mar2811580077832
Quantity: Over 20 available
Seller: Ria Christie Collections, Uxbridge, United Kingdom
Condition: New. In. Seller Inventory # ria9781588291516_new
Quantity: Over 20 available
Seller: moluna, Greven, Germany
Gebunden. Condition: New. Includes supplementary material: sn.pub/extrasA comprehensive collection of readily reproducible techniques for the manipulation of recombinant plasmids using the bacterial host E. coli. The authors describe proven methods for cloning DNA into pl. Seller Inventory # 458519153
Quantity: Over 20 available