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Synopsis
Leading scientists in gene expression methodology and bioinformatics data analysis describe readily reproducible methods for measuring RNA levels in cells and tissues. The techniques presented include new methods for applying the Affymetrix GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management. By distilling the basic underlying principles of many methods to a few straightforward concepts, investigators can easily choose the method most appropriate to their application.
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From the Back Cover
The transcription of messenger RNA from a DNA template is a key process in a wide variety of biological systems. In Gene Expression Profiling: Methods and Protocols, leaders in gene expression methodology and bioinformatics data analysis share their best methods for measuring RNA levels in cells and tissues. Each proven protocol is described in step-by-step detail and contains an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. Special care has been taken to distill the basic underlying principles of each method to a few straightforward concepts so that investigators can easily choose the method most appropriate to their application. The readily reproducible techniques presented include new methods for applying the GeneChip®, SAR-SAGE, StaRT-PCR, SSH, the Invader Assay®, and ADGEM. The authors also provide critical bioinformatics insight and resources for data analysis and management.
Cutting-edge and highly practical, Gene Expression Profiling: Methods and Protocols allows novice and experienced investigators alike not only to choose the best method to determine how genes are turned on and off in many different organisms, but also to use informatics resources to analyze and understand the results.
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- PublisherHumana
- Publication date2004
- ISBN 10 1588292207
- ISBN 13 9781588292209
- BindingHardcover
- LanguageEnglish
- Number of pages178
- EditorShimkets Richard A.
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Gene Expression Profiling (Methods in Molecular Biology, 258)
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Gene Expression Profiling: Methods and Protocols
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ISBN 10: 1588292207
ISBN 13: 9781588292209
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GENE EXPRESSION PROFILING. Methods and Protocols.
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Humana Press, Totowa NJ 2004., 2004
ISBN 10: 1588292207
ISBN 13: 9781588292209
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