Recombinant Gene Expression: Reviews and Protocols

Balbas, Paulina; Lorence, Argelia

ISBN 10: 1592597742 ISBN 13: 9781592597741

Publisher: Humana Press Inc., 2014

Synopsis

Leading investigators, all of them experts on particular gene expression systems, provide a stellar set of original protocols for gene expression supported by overviews and troubleshooting guides for the biological systmes addressed. This book provides a global view of gene expression in prokaryotes, fungi, plants and animals and will be an invaluable reference in the collection of all biological science laboratories.

About this title

Review

From reviews of the first edition...
"...particularly useful for teaching molecular biology laboratory courses."
-Quarterly Review of Biology

"...represents good value and will be a useful addition to the laboratory."
-Microbiology Today

From the Back Cover

In this updated and expanded second edition of an established classic, the editors have added critical reviews to a fresh collection of cutting-edge protocols for gene expression in bacteria, fungi, plants, plant cells, animals, and animal cells. The review articles survey new directions in recombinant gene expression research, technique, and application, and point the way to using recombinant gene expression for metabolic engineering and the production of nonprotein molecules. The readily reproducible protocols offer new host-vector systems, such as Antarctic bacteria and moderately halophytic bacteria, vectors for chromosomal editing and metabolic engineering, and information to aid in problem solving, strategy planning, and the promotion of protein folding. There are also novel heterologous gene expression schemes for cell-free systems, plant chloroplasts, microplates, hairy roots, and animal milk, and new developments in consolidated heterologous systems. The protocols follow the successful Methods in Molecular Biology™ series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
State-of-the-art and forward looking, Recombinant Gene Expression: Reviews and Protocols, Second Edition offers investigators seeking an overview of this critically important field not only the understanding, but also the tools needed to begin producing nonprotein products and altering the central metabolic pathways of cells to enhance heterologous gene expression.

Edition details

• Publisher: Humana Press Inc.
• Publication date: 2014
• Language: English
• ISBN 10: 1592597742
• ISBN 13: 9781592597741
• Binding: CD-ROM
• Edition number: 2
• Number of pages: 535
• Editor: Balbas Paulina, Lorence Argelia