Manipulating the Mouse Embryo: A Laboratory Manual, Fourth edition

0 avg rating
( 0 ratings by Goodreads )
 
9781936113002: Manipulating the Mouse Embryo: A Laboratory Manual, Fourth edition
View all copies of this ISBN edition:
 
 

This 4th edition of the "Mouse Manual"-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. While many of the techniques described in earlier editions of this manual have been relegated to core facilities, advances in DNA sequencing techniques and genome analysis have opened new avenues of research and developments. New approaches, such as the derivation of induced pluripotent stem cells and new targeted gene manipulation techniques that enable direct injection of RNA/DNA constructs into zygotes to achieve gene targeting, require new explanations and protocols. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have taken these developments into account in updating and rewriting this fourth edition to include new information and protocols on:

· generation of induced pluripotent stem cells
· RNA microinjections
· lentiviral microinjections and infection
· assisted reproduction techniques for sperm and embryo cryopreservation
· isolation, generation, and transplantation of spermatogonial stem cell lines
· in utero electroporation of gene constructs into postimplantation embryos
· vibratome sectioning of live and fixed tissues for imaging thick tissue sections
· whole-mount fluorescent staining methods for three-dimensional visualization

In addition, the wealth of essential information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups has been entirely updated. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. The field's technological sophistication has grown exponentially since then, but the manual remains the essential practical and theoretical guide for all students, lab technicians, and investigators who work with mice.

I have the third edition. Why should I buy the fourth edition?
The new technologies regarding induced pluripotent stem cells and RNA injection and CRISPR technology are changing the field and opening up new avenues of investigation. The protocols for these as well as other up-to-the minute techniques in the fourth edition are by the leading proponents in the field and offer clear step-by-step guidance with copious illustrations.

To whom is this edition addressed-the neophyte or the experienced investigator?
Both groups can benefit from this manual. For the neophyte, the summary of mouse development and the how-to's of setting up a working laboratory are an invaluable introduction to the field. For the experienced investigator, the detailed protocols of new techniques in the field, with extensive troubleshooting and over-the-shoulder advice, allow easy integration of these techniques into a laboratory's research objectives.
Are the protocols in this book better than those available for download on the Web?
Many of the protocols available on the Web are perfectly good, but there are many that are not. Reagents are expensive and deadlines are tight. CSHL Press manuals provide protocols that have been formulated and tested in the labs of leading investigators in the field. They are reliable and they work. And they include the context and troubleshooting information that many online protocols do not have. In the case of the protocols in this manual, there is a bit of "art" required in the performance of these protocols. But the experience and advice of these authors provides all of the information needed to achieve success in the lab.

"synopsis" may belong to another edition of this title.

Review:


"The editors...should be praised once again for a brilliant work done, accommodating the latest techniques in mouse embryo manipulation, while not forgetting the traditional procedures that must be learnt properly by anyone joining this field of animal transgenesis for the first time." ---International Society for Transgenic Technologies Blog


"About this title" may belong to another edition of this title.

Buy New View Book
List Price: US$ 240.00
US$ 216.17

Convert currency

Shipping: US$ 3.27
Within U.S.A.

Destination, rates & speeds

Add to Basket

Other Popular Editions of the Same Title

9781936113019: Manipulating the Mouse Embryo: A Laboratory Manual, Fourth edition

Featured Edition

ISBN 10:  1936113015 ISBN 13:  9781936113019
Publisher: Cold Spring Harbor Laboratory Press, 2013
Softcover

Top Search Results from the AbeBooks Marketplace

1.

Richard Behringer; Marina Gertsenstein; Kristina Nagy; Andras Nagy
Published by Cold Spring Harbor Laboratory Press (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 1
Seller:
Irish Booksellers
(Portland, ME, U.S.A.)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Press, 2013. Condition: New. book. Seller Inventory # M1936113007

More information about this seller | Contact this seller

Buy New
US$ 216.17
Convert currency

Add to Basket

Shipping: US$ 3.27
Within U.S.A.
Destination, rates & speeds

2.

Behringer, Richard; Marina Gertsenstein , Marina Gertsenstein ; Kristina Nagy , Kristina Nagy ; Andras Nagy, Andras Nagy
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Quantity Available: 5
Seller:
GreatBookPrices
(Columbia, MD, U.S.A.)
Rating
[?]

Book Description Condition: New. Seller Inventory # 19837256-n

More information about this seller | Contact this seller

Buy New
US$ 226.73
Convert currency

Add to Basket

Shipping: US$ 2.64
Within U.S.A.
Destination, rates & speeds

3.

Nagy
Published by Oxford University Press
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Quantity Available: 17
Seller:
INDOO
(Avenel, NJ, U.S.A.)
Rating
[?]

Book Description Oxford University Press. Condition: New. Brand New. Seller Inventory # 1936113007

More information about this seller | Contact this seller

Buy New
US$ 225.78
Convert currency

Add to Basket

Shipping: US$ 3.60
Within U.S.A.
Destination, rates & speeds

4.

Marina Gertsenstein, Kristina Nagy, Richard Behringer
Published by Cold Spring Harbor Laboratory Press,U.S., United States (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover First Edition Quantity Available: 10
Seller:
Book Depository hard to find
(London, United Kingdom)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Press,U.S., United States, 2013. Hardback. Condition: New. Lab Manual. Language: English. Brand new Book. This 4th edition of the "Mouse Manual"-Manipulating the Mouse Embryo-appears 28 years after the first edition and once again is the definitive reference source on mouse development, transgenesis techniques, and molecular biology. Authors Richard Behringer, Marina Gertsenstein, Kristina Nagy, and Andras Nagy-pre-emininent leaders in their fields-have reorganized and updated this edition to include new information and protocols on: * assisted reproduction techniques for sperm and embryo cryopreservation * generation of induced pluripotent stem cells * isolation, generation, and transplantation of spermatogonial stem cell lines * in utero electroporation of gene constructs into post- implantation embryos * vibratome sectioning of live and fixed tissues for imaging thick tissue sections * whole-mount fluorescent staining methods for three-dimensional visualization. Techniques regarding recombinant DNA technology and mouse embryonic development from the previous editions have been updated and recast, as has the wealth of information on mouse laboratory strains, mouse housing and breeding, surgical procedures, assisted reproduction, handling of embryos, and micromanipulation setups. The first edition of Manipulating the Mouse Embryo appeared in 1986 as an outgrowth of Cold Spring Harbor Laboratory courses on the molecular embryology of the mouse held in the early 1980s, and authors of the first two editions included Brigid Hogan, Rosa Beddington, Frank Costantini, and Liz Lacy. Mouse embryo manipulation techniques have developed exponentially since the first edition, but then, as now, Manipulating the Mouse Embryo remains the essential practical and theoretical guide for anyone working with mice-students, lab technicians, and investigators. Seller Inventory # BTE9781936113002

More information about this seller | Contact this seller

Buy New
US$ 294.38
Convert currency

Add to Basket

Shipping: FREE
From United Kingdom to U.S.A.
Destination, rates & speeds

5.

Richard, PH.D. Behringer; Marina (Toronto Centre for Phengenomics, Transgenic Core and Specialty Resources) Gertsenstein; Kristina (Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto) Nagy; Andras Nagy
Published by Cold Spring Harbor Laboratory Press,U.S. (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 2
Seller:
Rating
[?]

Book Description Cold Spring Harbor Laboratory Press,U.S., 2013. Condition: New. Seller Inventory # TV9781936113002

More information about this seller | Contact this seller

Buy New
US$ 366.58
Convert currency

Add to Basket

Shipping: US$ 3.21
From Germany to U.S.A.
Destination, rates & speeds

6.

Richard Behringer
Published by Cold Spring Harbor Laboratory Press (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 1
Seller:
Books Express
(Portsmouth, NH, U.S.A.)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Press, 2013. Hardcover. Condition: New. 4 Lab. Ships with Tracking Number! INTERNATIONAL WORLDWIDE Shipping available. Buy with confidence, excellent customer service!. Seller Inventory # 1936113007n

More information about this seller | Contact this seller

Buy New
US$ 472.37
Convert currency

Add to Basket

Shipping: FREE
Within U.S.A.
Destination, rates & speeds

7.

Behringer, Richard/ Gertsenstein, Marina/ Nagy, Kristina/ Nagy, Andras
Published by Cold Spring Harbor Laboratory Pr (2013)
ISBN 10: 1936113007 ISBN 13: 9781936113002
New Hardcover Quantity Available: 1
Seller:
Revaluation Books
(Exeter, United Kingdom)
Rating
[?]

Book Description Cold Spring Harbor Laboratory Pr, 2013. Hardcover. Condition: Brand New. 4th lab edition. 814 pages. 11.25x9.00x1.75 inches. In Stock. Seller Inventory # __1936113007

More information about this seller | Contact this seller

Buy New
US$ 472.66
Convert currency

Add to Basket

Shipping: US$ 9.86
From United Kingdom to U.S.A.
Destination, rates & speeds