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Synopsis
2-氧-D-吡喃型葡萄糖基-L-抗坏血酸（AA-2G）是一种维生素C的糖基化衍生物。因其在水溶液中具有较强的抗氧化性和稳定性,被广泛应用在食品、化妆品、医药等领域。目前AA-2G生产方法主要为酶转化法，由于α-和β-环糊精具有较高的转化率，常被用作糖基供体，在环糊精葡萄糖基转移酶（CGTase）催化作用下合成AA-2G。然而，由于α-环糊精价格昂贵，β-环糊精很难溶于水，所以从生产成本考虑，两者均不适合大规模生产AA-2G。本书以Paenibacillus macerans来源的CGTase为研究对象，分别通过不同的基因工程及蛋白质工程策略（主要包括定点饱和突变、组合饱和突变、迭代饱和突变、结构域嵌合及短肽融合方法）分别获得不同的CGTase优良突变体，通过酶学性质分析，发现这些突变酶以麦芽糊精或可溶性淀粉为底物合成AA-2G的转化率均有提高。并且通过蛋白结构模拟对突变酶的结构进行了分析，进一步对其催化机理进行了预测。本书不仅为降低AA-2G生产成本提供了思路，同时也对其他类似酶的蛋白质工程改造具有一定的借鉴意义。
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About the Author
韩瑞枝，男，江南大学副教授，硕士生导师。分别在合肥工业大学，四川大学，江南大学获得工学学士，硕士，博士学位。博士期间在Biotechnol Adv、Appl Environ Microbiol、Appl Microbiol Biotechnol等国际著名期刊以第一作者发表多篇论文，并荣获2015年江苏省优秀博士论文。目前主持项目包括国家自然科学基金和江苏省自然科学基金。研究方向主要为酶工程及蛋白质工程领域。
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huan hu jing pu tao tang ji zhuan yi mei de fen zi gai zao ji he cheng tang ji hua L kang huai xie suan
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -2 yang D bi nan xing pu tao tang ji L kang huai xie suan AA 2G shi yi zhong wei sheng su C de tang ji hua yan sheng wu yin qi zai shui rong ye zhong ju you jiao qiang de kang yang hua xing he wen ding xing bei guang fan ying yong zai shi pin hua zhuang pin yi yao deng ling yu mu qian AA 2G sheng chan fang fa zhu yao wei mei zhuan hua fa you yu he huan hu jing ju you jiao gao de zhuan hua lv chang bei yong zuo tang ji gong ti zai huan hu jing pu tao tang ji zhuan yi mei CGTase cui hua zuo yong xia he cheng AA 2G ran er you yu huan hu jing jia ge ang gui huan hu jing hen nan rong yu shui suo yi cong sheng chan cheng ben kao lv liang zhe jun bu shi he da gui mo sheng chan AA 2G ben shu yi Paenibacillus macerans lai yuan de CGTase wei yan jiu dui xiang fen bie tong guo bu tong de ji yin gong cheng ji dan bai zhi gong cheng ce lve zhu yao bao kuo ding dian bao he tu bian zu he bao he tu bian die dai bao he tu bian jie gou yu qian he ji duan tai rong he fang fa fen bie huo de bu tong de CGTase you liang tu bian ti tong guo mei xue xing zhi fen xi fa xian zhe xie tu bian mei yi mai ya hu jing huo ke rong xing dian fen wei di wu he cheng AA 2G de zhuan hua lv jun you ti gao bing qie tong guo dan bai jie gou mo ni dui tu bian mei de jie gou jin xing le fen xi jin yi bu dui qi cui hua ji li jin xing le yu ce ben shu bu jin wei jiang di AA 2G sheng chan cheng ben ti gong le si lu tong shi ye dui qi ta lei si mei de dan bai zhi gong cheng gai zao ju you yi ding de jie jian yi yi 132 pp. Chinesisch. Seller Inventory # 9783330823662
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huan hu jing pu tao tang ji zhuan yi mei de fen zi gai zao ji he cheng tang ji hua L kang huai xie suan
Print on DemandSeller: AHA-BUCH GmbH, Einbeck, Germany
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - 2 yang D bi nan xing pu tao tang ji L kang huai xie suan AA 2G shi yi zhong wei sheng su C de tang ji hua yan sheng wu yin qi zai shui rong ye zhong ju you jiao qiang de kang yang hua xing he wen ding xing bei guang fan ying yong zai shi pin hua zhuang pin yi yao deng ling yu mu qian AA 2G sheng chan fang fa zhu yao wei mei zhuan hua fa you yu he huan hu jing ju you jiao gao de zhuan hua lv chang bei yong zuo tang ji gong ti zai huan hu jing pu tao tang ji zhuan yi mei CGTase cui hua zuo yong xia he cheng AA 2G ran er you yu huan hu jing jia ge ang gui huan hu jing hen nan rong yu shui suo yi cong sheng chan cheng ben kao lv liang zhe jun bu shi he da gui mo sheng chan AA 2G ben shu yi Paenibacillus macerans lai yuan de CGTase wei yan jiu dui xiang fen bie tong guo bu tong de ji yin gong cheng ji dan bai zhi gong cheng ce lve zhu yao bao kuo ding dian bao he tu bian zu he bao he tu bian die dai bao he tu bian jie gou yu qian he ji duan tai rong he fang fa fen bie huo de bu tong de CGTase you liang tu bian ti tong guo mei xue xing zhi fen xi fa xian zhe xie tu bian mei yi mai ya hu jing huo ke rong xing dian fen wei di wu he cheng AA 2G de zhuan hua lv jun you ti gao bing qie tong guo dan bai jie gou mo ni dui tu bian mei de jie gou jin xing le fen xi jin yi bu dui qi cui hua ji li jin xing le yu ce ben shu bu jin wei jiang di AA 2G sheng chan cheng ben ti gong le si lu tong shi ye dui qi ta lei si mei de dan bai zhi gong cheng gai zao ju you yi ding de jie jian yi yi. Seller Inventory # 9783330823662
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Seller: Revaluation Books, Exeter, United Kingdom
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Paperback. Condition: Brand New. 132 pages. Chinese language. 8.66x5.91x0.30 inches. In Stock. Seller Inventory # 3330823666
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huan hu jing pu tao tang ji zhuan yi mei de fen zi gai zao ji he cheng tang ji hua L kang huai xie suan
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Über den Autorrnrnhan rui zhi nan jiang nan da xue fu jiao shou shuo shi sheng dao shi fen bie zai he fei gong ye da xue si chuan da xue jiang nan da xue huo de gong xue xue shi shuo shi bo shi xue wei bo shi qi jian zai Biotechnol. Seller Inventory # 510068366
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huan hu jing pu tao tang ji zhuan yi mei de fen zi gai zao ji he cheng tang ji hua L kang huai xie suan
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Taschenbuch. Condition: Neu. Neuware -2 yang D bi nan xing pu tao tang ji L kang huai xie suan AA 2G shi yi zhong wei sheng su C de tang ji hua yan sheng wu yin qi zai shui rong ye zhong ju you jiao qiang de kang yang hua xing he wen ding xing bei guang fan ying yong zai shi pin hua zhuang pin yi yao deng ling yu mu qian AA 2G sheng chan fang fa zhu yao wei mei zhuan hua fa you yu he huan hu jing ju you jiao gao de zhuan hua lv chang bei yong zuo tang ji gong ti zai huan hu jing pu tao tang ji zhuan yi mei CGTase cui hua zuo yong xia he cheng AA 2G ran er you yu huan hu jing jia ge ang gui huan hu jing hen nan rong yu shui suo yi cong sheng chan cheng ben kao lv liang zhe jun bu shi he da gui mo sheng chan AA 2G ben shu yi Paenibacillus macerans lai yuan de CGTase wei yan jiu dui xiang fen bie tong guo bu tong de ji yin gong cheng ji dan bai zhi gong cheng ce lve zhu yao bao kuo ding dian bao he tu bian zu he bao he tu bian die dai bao he tu bian jie gou yu qian he ji duan tai rong he fang fa fen bie huo de bu tong de CGTase you liang tu bian ti tong guo mei xue xing zhi fen xi fa xian zhe xie tu bian mei yi mai ya hu jing huo ke rong xing dian fen wei di wu he cheng AA 2G de zhuan hua lv jun you ti gao bing qie tong guo dan bai jie gou mo ni dui tu bian mei de jie gou jin xing le fen xi jin yi bu dui qi cui hua ji li jin xing le yu ce ben shu bu jin wei jiang di AA 2G sheng chan cheng ben ti gong le si lu tong shi ye dui qi ta lei si mei de dan bai zhi gong cheng gai zao ju you yi ding de jie jian yi yiVDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 132 pp. Chinesisch. Seller Inventory # 9783330823662
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