EFFECT OF DIFFERENT GROUP OF PESTICIDES ON LESSER GRAIN BORER: Effect of Different Group of Pesticides on Zymogram Against Lesser Grain Borer (Rhyzopertha dominica) - Softcover

Azmi, Dr. M Arshad; Shabbir, Sobia

Softcover

ISBN 10: 3639321626 ISBN 13: 9783639321623

Publisher: VDM Verlag Dr. Müller, 2010

Synopsis

Adults of Rhyzopertha dominica were treated by filter paper impregnation method with five doses of pesticides of different groups such as cypermethrin, lorsban, Acorus calamus extract, trend and shogun, and LC50 was calculated. Biochemical analysis revealed that after treatment with LC50 of pesticides, cypermethrin inhibited 80% alkaline phosphatase activity, lorsban 44.17%, A. calamus extract 49.87%, trend 25% and shogun inhibited 20% activity as compared to control. Acid phosphatase activity was inhibited upto 42.8%, 11.12%, 14.25%, 28.62% and 33%, respectively. Similarly, cholinesterase activity was inhibited upto 60%, 20%, 33.33%, 50% and 59.2%, respectively. Total protein content was inhibited i.e., 29.27%, 20.49%, 20.49%, 64.76% and 41.19%, respectively. Thin layer chromatography of protein metabolites of R. dominica was found to be slightly different in the treated and non-treated insect and change in the protein peptide pattern was observed. Polyacrylamide gel electrophoresis was also done for localization of protein bands in treated samples as compared to control. This information is useful for pest management worker and researchers in this field as well for teachers.

About this title

About the Author

Prof Dr M Arshad Azmi, Curriculum Vitae: Specialization: Entomology Qualification: Ph.D. Teaching and Research experience: 23 years, 95 research publications, Administrative experience: 10 years, Working as Professor in Department of Zoology, University of Karachi. Member of many academic and scientific bodies.

Edition details

• Publisher: VDM Verlag Dr. Müller
• Publication date: 2010
• ISBN 10: 3639321626
• ISBN 13: 9783639321623
• Binding: Paperback
• Language: English
• Number of pages: 152