Uricase is an important enzyme in the metabolism of purines catalyzing the oxidation of uric acid in the presence of oxygen, producing allantion, H2O2 and CO2. In humans and great apes, uric acid is the final product of the purine catabolism pathway - lower organisms express uricase which further degrades uric acid, however due to nonsense mutations in the urate oxidase gene (UOX pseudogene) it is not expressed in humans. Uricase enzyme was isolated from bovine kidney. The activity of crud uricase noted was3721.04 U/mL and 135.681 U/mg specific activity with 27.426 mg/mL of protein contents. The activity 9581.843 U/mL and specific activity 534.641 U/mg were obtained when enzyme was subjected to ammonium sulfate precipitation technique at 50% saturation. The rate of irreversible thermal denaturation was determined by incubating the Uricase at different temperatures ranging from 37 – 60°C. Half-life decreased from 660 to 149.4 min after heating at 60°C as compared to 37°C. The enzyme had positive ∆S*, ∆H* and ∆G*.
"synopsis" may belong to another edition of this title.
Dr. Munazzah Meraj, PhD Biochemistry, studied at University of Agriculture, Faisalabad. Assistant Professor at Peoples University of Medical and Health Sciences, Nawabshah, Pakistan.
"About this title" may belong to another edition of this title.
Shipping:
US$ 25.74
From Germany to U.S.A.
Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Uricase is an important enzyme in the metabolism of purines catalyzing the oxidation of uric acid in the presence of oxygen, producing allantion, H2O2 and CO2. In humans and great apes, uric acid is the final product of the purine catabolism pathway - lower organisms express uricase which further degrades uric acid, however due to nonsense mutations in the urate oxidase gene (UOX pseudogene) it is not expressed in humans. Uricase enzyme was isolated from bovine kidney. The activity of crud uricase noted was3721.04 U/mL and 135.681 U/mg specific activity with 27.426 mg/mL of protein contents. The activity 9581.843 U/mL and specific activity 534.641 U/mg were obtained when enzyme was subjected to ammonium sulfate precipitation technique at 50% saturation. The rate of irreversible thermal denaturation was determined by incubating the Uricase at different temperatures ranging from 37 60°C. Half-life decreased from 660 to 149.4 min after heating at 60°C as compared to 37°C. The enzyme had positive S , H and G . 96 pp. Englisch. Seller Inventory # 9783639705881
Quantity: 2 available
Seller: AHA-BUCH GmbH, Einbeck, Germany
Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Uricase is an important enzyme in the metabolism of purines catalyzing the oxidation of uric acid in the presence of oxygen, producing allantion, H2O2 and CO2. In humans and great apes, uric acid is the final product of the purine catabolism pathway - lower organisms express uricase which further degrades uric acid, however due to nonsense mutations in the urate oxidase gene (UOX pseudogene) it is not expressed in humans. Uricase enzyme was isolated from bovine kidney. The activity of crud uricase noted was3721.04 U/mL and 135.681 U/mg specific activity with 27.426 mg/mL of protein contents. The activity 9581.843 U/mL and specific activity 534.641 U/mg were obtained when enzyme was subjected to ammonium sulfate precipitation technique at 50% saturation. The rate of irreversible thermal denaturation was determined by incubating the Uricase at different temperatures ranging from 37 60°C. Half-life decreased from 660 to 149.4 min after heating at 60°C as compared to 37°C. The enzyme had positive S , H and G . Seller Inventory # 9783639705881
Quantity: 1 available
Seller: moluna, Greven, Germany
Kartoniert / Broschiert. Condition: NEW. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Meraj MunazzahDr. Munazzah Meraj, PhD Biochemistry, studied at University of Agriculture, Faisalabad. Assistant Professor at Peoples University of Medical and Health Sciences, Nawabshah, Pakistan.Uricase is an important enzyme in. Seller Inventory # 4998976
Quantity: Over 20 available
Seller: Revaluation Books, Exeter, United Kingdom
Paperback. Condition: NEW. 96 pages. 8.66x5.91x0.22 inches. In Stock. Seller Inventory # __3639705882
Quantity: 1 available
Seller: Revaluation Books, Exeter, United Kingdom
Paperback. Condition: NEW. 96 pages. 8.66x5.91x0.22 inches. In Stock. Seller Inventory # 3639705882
Quantity: 1 available