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Microorganisms, which secret a complex of mycolytic enzymes are considered to be possible biological control agents of plant diseases.In this present study, an industrial enzyme chitinase produced by Bacillus species was optimized, purified and its antifungal activity was investigated against plant pathogens. The bacteria Bacillus species was isolated from soil sample and it was identified by staining techniques, plating on selective media and biochemical characteristics. The Bacillus species showed highest chitinolytic activity in Colloidal chitin agars that degrade chitin in 0.6 mm zone of clearance. The production of chitinase by Bacillus species was optimized under different media, substrates, substrate concentrations, pH, temperature and incubation period. The maximum chitinase production was observed in Luria Bertaini Broth amended with 0.3% colloidal chitin at pH 7.0 and temperature 37˚C after four days of incubation. The enzyme was partially purified by Dialysis method. The Chitinase has antifungal activity against plant pathogens viz, Aspergillus niger, Aspergillus flavus and Penicillium chrysogenum.
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Mr.S.Kavi Karunya is a Ph.D Scholar in Department of Microbiology, Annamalai University, Chidambaram, Tamilnadu, India. He published many papers in International journals & attended several national & international level seminars & conferences. He published one monograph book in LAP, Germany. His area of specification is Agricultural Microbiology.
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- PublisherLAP LAMBERT Academic Publishing
- Publication date2014
- ISBN 10 3659575755
- ISBN 13 9783659575754
- BindingPaperback
- Number of pages72
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Book Description Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Microorganisms, which secret a complex of mycolytic enzymes are considered to be possible biological control agents of plant diseases.In this present study, an industrial enzyme chitinase produced by Bacillus species was optimized, purified and its antifungal activity was investigated against plant pathogens. The bacteria Bacillus species was isolated from soil sample and it was identified by staining techniques, plating on selective media and biochemical characteristics. The Bacillus species showed highest chitinolytic activity in Colloidal chitin agars that degrade chitin in 0.6 mm zone of clearance. The production of chitinase by Bacillus species was optimized under different media, substrates, substrate concentrations, pH, temperature and incubation period. The maximum chitinase production was observed in Luria Bertaini Broth amended with 0.3% colloidal chitin at pH 7.0 and temperature 37 C after four days of incubation. The enzyme was partially purified by Dialysis method. The Chitinase has antifungal activity against plant pathogens viz, Aspergillus niger, Aspergillus flavus and Penicillium chrysogenum. 72 pp. Englisch. Seller Inventory # 9783659575754
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Book Description Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Microorganisms, which secret a complex of mycolytic enzymes are considered to be possible biological control agents of plant diseases.In this present study, an industrial enzyme chitinase produced by Bacillus species was optimized, purified and its antifungal activity was investigated against plant pathogens. The bacteria Bacillus species was isolated from soil sample and it was identified by staining techniques, plating on selective media and biochemical characteristics. The Bacillus species showed highest chitinolytic activity in Colloidal chitin agars that degrade chitin in 0.6 mm zone of clearance. The production of chitinase by Bacillus species was optimized under different media, substrates, substrate concentrations, pH, temperature and incubation period. The maximum chitinase production was observed in Luria Bertaini Broth amended with 0.3% colloidal chitin at pH 7.0 and temperature 37 C after four days of incubation. The enzyme was partially purified by Dialysis method. The Chitinase has antifungal activity against plant pathogens viz, Aspergillus niger, Aspergillus flavus and Penicillium chrysogenum. Seller Inventory # 9783659575754
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Book Description Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: S. Kavi KarunyaMr.S.Kavi Karunya is a Ph.D Scholar in Department of Microbiology, Annamalai University, Chidambaram, Tamilnadu, India. He published many papers in International journals & attended several national & international lev. Seller Inventory # 5165922
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