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Synopsis
Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis method (SBS) - should be able to detect DNA mutations faster and more accurate than the currently available sequencing technique. The function of a reversible terminator used for SBS is as follows: 3?-modified, dye-labeled nucleotides are incorporated by the polymerase complementary to the DNA-template (as already known from Sanger sequencing) stopping the DNA-synthesis immediately. After detection of the fluorescent signal, the reversible terminator has to be cleavable in such a way (here: the 3?-modification) that the DNA-synthesis can continue (i. e. ?reversible? termination). Thus SBS enables a step-by-step chain elongation with a direct read-out of the sequence via fluorescent signals. This PhD thesis covers the synthesis of 3?-modified nucleosides as key compounds for these terminators and the evaluation of suitable 3?-modification cleavage conditions.
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About the Author
Angelika C. Keller was born in Erlangen/Germany in 1979 and studied chemistry from 2000 to 2006 in Frankfurt/Main. She received her diploma in 2006 with the topic ?Synthesis of reversible terminators for SBS? and continued the research on this subject during her PhD thesis which she finished in 2009.
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- PublisherSuedwestdeutscher Verlag fuer Hochschulschriften
- Publication date2010
- ISBN 10 3838113551
- ISBN 13 9783838113555
- BindingPaperback
- LanguageEnglish
- Number of pages240
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Synthesis and enzymatic testing of reversible terminators for SBS
Published by
Südwestdeutscher Verlag Für Hochschulschriften AG Co. KG Okt 2015, 2015
ISBN 10: 3838113551
ISBN 13: 9783838113555
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Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis method (SBS) - should be able to detect DNA mutations faster and more accurate than the currently available sequencing technique. The function of a reversible terminator used for SBS is as follows: 3 -modified, dye-labeled nucleotides are incorporated by the polymerase complementary to the DNA-template (as already known from Sanger sequencing) stopping the DNA-synthesis immediately. After detection of the fluorescent signal, the reversible terminator has to be cleavable in such a way (here: the 3 -modification) that the DNA-synthesis can continue (i. e. reversible termination). Thus SBS enables a step-by-step chain elongation with a direct read-out of the sequence via fluorescent signals. This PhD thesis covers the synthesis of 3 -modified nucleosides as key compounds for these terminators and the evaluation of suitable 3 -modification cleavage conditions. 240 pp. Englisch. Seller Inventory # 9783838113555
Quantity: 2 available
Seller Image
Synthesis and enzymatic testing of reversible terminators for SBS
Published by
Südwestdeutscher Verlag Für Hochschulschriften AG Co. KG, 2010
ISBN 10: 3838113551
ISBN 13: 9783838113555
New
Taschenbuch
Seller: AHA-BUCH GmbH, Einbeck, Germany
Seller rating 5 out of 5 stars
Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by-synthesis method (SBS) - should be able to detect DNA mutations faster and more accurate than the currently available sequencing technique. The function of a reversible terminator used for SBS is as follows: 3 -modified, dye-labeled nucleotides are incorporated by the polymerase complementary to the DNA-template (as already known from Sanger sequencing) stopping the DNA-synthesis immediately. After detection of the fluorescent signal, the reversible terminator has to be cleavable in such a way (here: the 3 -modification) that the DNA-synthesis can continue (i. e. reversible termination). Thus SBS enables a step-by-step chain elongation with a direct read-out of the sequence via fluorescent signals. This PhD thesis covers the synthesis of 3 -modified nucleosides as key compounds for these terminators and the evaluation of suitable 3 -modification cleavage conditions. Seller Inventory # 9783838113555
Quantity: 1 available
Seller Image
Synthesis and enzymatic testing of reversible terminators for SBS
Published by
Südwestdeutscher Verlag für Hochschulschriften, 2015
ISBN 10: 3838113551
ISBN 13: 9783838113555
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Seller: moluna, Greven, Germany
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Based on the state-of-the-art DNA sequencing method - Sanger sequencing - the major aim of future research is to develop a cost-effective and gel electrophoresis-free sequencing method. These new sequencing technologies - such as the so-called sequencing-by. Seller Inventory # 5405731
Quantity: Over 20 available