Application of Microarray technology: A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation. - Softcover
Synopsis
The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions.
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Application of Microarray technology
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions. 68 pp. Englisch. Seller Inventory # 9783838364063
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Application of Microarray technology
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Ray AnamikaAnamika Ray, PhD, Oklahoma State University, Working as a Postdoctoral Research Scientist in the Dept. of Biochemistry and Molecular Biology at Oklahoma State University. Co-Authors: P.Ayoubi, R.Prade, A.J. Mort.The co. Seller Inventory # 5416747
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Application of Microarray technology
Published by
LAP LAMBERT Academic Publishing Mai 2010, 2010
ISBN 10: 3838364066
ISBN 13: 9783838364063
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Taschenbuch. Condition: Neu. Neuware -The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions.Books on Demand GmbH, Überseering 33, 22297 Hamburg 68 pp. Englisch. Seller Inventory # 9783838364063
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Application of Microarray technology : A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation.
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ISBN 13: 9783838364063
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The construction of cDNA libraries is useful in understanding gene expression in organisms under different conditions, but random sequencing of unbiased cDNAs is laborious and gives rise to redundancy. We aimed to isolate cDNAs induced by switching Aspergillus nidulans from glucose to selected polysaccharides. We devised a Negative Subtraction Hybridization method which involved screening a library made from cDNAs from cells grown under a selected physiological condition with labeled cDNA probes from another physiological condition. In a screen for transcripts induced by switching the carbon source, 3,532 negatives were isolated. Single-channel microarray experiments were done to confirm that the majority of the negatives were indeed induced differentially. To gain insight into the regulation, we used microarrays to identify gene expression changes following a shift from glucose to pectin or glucose starvation. The findings from this study portray the useful utilization of the powerful microarray technology to study gene expression profiling under various physiological conditions. Seller Inventory # 9783838364063
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Application of Microarray technology | A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation.
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Taschenbuch. Condition: Neu. Application of Microarray technology | A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation. | Anamika Ray | Taschenbuch | 68 S. | Englisch | 2010 | LAP LAMBERT Academic Publishing | EAN 9783838364063 | Verantwortliche Person für die EU: BoD - Books on Demand, In de Tarpen 42, 22848 Norderstedt, info[at]bod[dot]de | Anbieter: preigu. Seller Inventory # 101106112
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Application of Microarray technology: A study of gene expression profiling in Aspergillus nidulans in pectin and glucose starvation.
Published by
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ISBN 13: 9783838364063
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