Items related to Expression and Characterization of Nucleocapsid (N)...
Synopsis
The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF’ cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
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LAP LAMBERT Academic Publishing Feb 2020, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Seller: BuchWeltWeit Ludwig Meier e.K., Bergisch Gladbach, Germany
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Taschenbuch. Condition: Neu. This item is printed on demand - it takes 3-4 days longer - Neuware -The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low. 64 pp. Englisch. Seller Inventory # 9786200587886
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Published by
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Taschenbuch
Seller: AHA-BUCH GmbH, Einbeck, Germany
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Taschenbuch. Condition: Neu. nach der Bestellung gedruckt Neuware - Printed after ordering - The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low. Seller Inventory # 9786200587886
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Published by
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Seller: moluna, Greven, Germany
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Condition: New. Dieser Artikel ist ein Print on Demand Artikel und wird nach Ihrer Bestellung fuer Sie gedruckt. Autor/Autorin: Shrivastava NeerajDr Neeraj Shrivastava (Ph.D) is working as Assistant Professor in the Department of Veterinary Microbiology at N.D.V.S.U.,Jabalpur(M.P.)The present work is his MVSc thesis.Dr Shrivastava is a gold medalist in B.V.Sc. Seller Inventory # 385895895
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Published by
LAP LAMBERT Academic Publishing Feb 2020, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
New
Taschenbuch
Seller: buchversandmimpf2000, Emtmannsberg, BAYE, Germany
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Taschenbuch. Condition: Neu. This item is printed on demand - Print on Demand Titel. Neuware -The book is MVSc research work of the author on recombinant plasmid containing N gene of rinderpest (pBK-CMV-39) of eukaryotic expression of N protein. The fusion protein region along with prokaryotic promoter was removed by Nhel-Spel digestion. The digested plasmid was religated and was used for transforming XL-1 Blue MRF' cells From the transformed cells the plasmid (pBK-CMV-NS) was purified and characterized by RE digestion. Both the plasmids were used for eukaryotic expression studies.While the cells transfected with pBK-CMV-G9 show expression of a protein of the size 60 kD which corresponds to size of N protein of RPV. The expressed protein reacted with rinderpest polyclonal serum in western blot and ELISA. This indicate that the expressed protein resembles N protein of RPV in immunogenicity. Localization by FAT showed expressed protein was predominantly localized in the cytoplasm of transfected cells. The Number transfected cells were very low.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 64 pp. Englisch. Seller Inventory # 9786200587886
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Published by
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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Taschenbuch
Seller: preigu, Osnabrück, Germany
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Taschenbuch. Condition: Neu. Expression and Characterization of Nucleocapsid (N) Gene of R.[.] | Neeraj Shrivastava (u. a.) | Taschenbuch | 64 S. | Englisch | 2020 | LAP LAMBERT Academic Publishing | EAN 9786200587886 | Verantwortliche Person für die EU: preigu GmbH & Co. KG, Lengericher Landstr. 19, 49078 Osnabrück, mail[at]preigu[dot]de | Anbieter: preigu. Seller Inventory # 118114926
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Expression and Characterization of Nucleocapsid (N) Gene of R.P.Virus
Published by
LAP LAMBERT Academic Publishing, 2020
ISBN 10: 6200587884
ISBN 13: 9786200587886
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paperback. Condition: New. NEW. SHIPS FROM MULTIPLE LOCATIONS. book. Seller Inventory # ERICA82962005878846
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