The book is divided into eight chapters. The first chapter introduces the core method of sequence comparison; The second chapter focuses on the analysis tool of nucleotide sequence, including: gene prediction, coding region structural analysis, the variable shear analysis, predicting gene promoter, gene regulatory elements prediction, codon preference analysis etc. The third chapter, protein amino acid sequence analysis starts from searching data bases. Then the book introduces the basic physicochemical properties, secondary structure, protein domain, three-dimensional structure and predict the biological function of the target protein. The fourth chapter use TM4 software to implement chip data acquisition, low-quality filtering and standardization. GenMAPP softwear helps discover chip datas biological meaning in metabolic pathway.
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