Laboratory Manual for Genetic Engineering

 
9788120338142: Laboratory Manual for Genetic Engineering
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The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology.

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Book Description 2009. Soft cover. Condition: New. Dust Jacket Condition: New. International Edition. Book Condition: BRAND NEW. International/Eastern Economy Edition, Paperback/Softcover with SAME TITLE, AUTHOR AND EDITION as listed. ISBN and Cover design differs. **100% IDENTICAL CONTENTS as U.S Edition**. Standard Delivery within 7-14 business days ACROSS THE GLOBE. We can ship to PO Box, APO address in US. International Edition Textbooks may bear a label (Not for sale in the U.S. or Canada) or (For sale in Asia only) or similar restrictions- printed only to discourage students from obtaining an affordable copy. US Court has asserted your right to buy and use International edition. Access code/CD may not provided with these editions. We may ship the books from MULTIPLE WAREHOUSES ACROSS THE GLOBE including Asia depending upon the availability of inventory. Printed in English. Customer satisfaction guaranteed. Seller Inventory # ABECA5279

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Book Description PHI Learning, 2009. Softcover. Condition: New. This systematically designed laboratory manual elucidates a number of techniques which help the students carry out various experiments in the field of genetic engineering. The book explains the methods for the isolation of DNA and RNA as well as electrophoresis techniques for DNA, RNA and proteins. It discusses DNA manipulation by restriction digestion and construction of recombinant DNA by ligation. Besides, the book focuses on various methodologies for DNA transformation and molecular hybridization. While discussing all these techniques, the book puts emphasis on important techniques such as DNA isolation from Gram positive bacteria including Bacillus sp., the slot-lysis electrophoresis technique which is useful in DNA profile analysis of both Gram negative and positive bacteria, plasmid transduction in Bacillus sp., and the conjugal transfer of plasmid DNA in cyanobacteria, Bacillus and Agrobacterium tumefaciens. This book is intended for the undergraduate and postgraduate students of biotechnology for their laboratory courses in genetic engineering. Besides, it will be useful for the students specializing in genetic engineering, molecular biology and molecular microbiology. CONTENTS: Preface 1. Isolation of Genomic DNA from Gram Negative, Gram Positive Bacteria, Blood and Mammalian Tissue 2. Isolation of Plasmid DNA from Gram Negative and Gram Positive Bacteria 3. Isolation of RNA from Bacteria and Cultured Mammalian Cells 4. Estimation of Nucleic Acids 5. Restriction Digestion and Ligation of DNA 6. Polymerase Chain Reaction and Randomly Amplified Polymorphic DNA 7. Electrophoresis of Nucleic Acids 8. Slot-lysis Agarose Gel Electrophoresis 9. Purification of DNA from Agarose and Polyacrylamide Gels 10. Transformation of Gram Negative and Gram Positive Bacteria with Plasmid DNA 11. Estimation of Proteins 12. Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis for Proteins 13. ?-Galactosidase Assay 14. Transduction of Plasmid DNA Using CP?51 and CP?54 Bacteriophages 15. Bacterial Conjugation 16. Blotting Techniques 17. 32P Labelled Probe Preparation and Measurement of Radioactivity in Radiolabelled Nucleic Acid 18. Hybridization Techniques Appendices Index Printed Pages: 131. Seller Inventory # 10664

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