Identification of Plant Viruses: Methods and Experiments - Softcover

Noordam, D.

 
9789022004647: Identification of Plant Viruses: Methods and Experiments

Synopsis

Dr Noordam's, despite its title, it is not a manual of virus identification. It describes a series of experiments and shows how they can be fitted into a 4-week course that emphasizes the plant pathological aspects of plant virology. The book includes experiments to obtain and record symptoms of infection, to determine some of the physical properties of virus particles, to purify them, and both to assay virus content and to transmit viruses from plant to plant in a variety of ways. Other sections deal with the interactions between viruses in plants, the separation of viruses or virus variants from mixed cultures, and methods of storing cultures. Most of the experiments are clearly described and there are many valuable practical hints, based on the author's experience. In some sections, there seems too little information on the basis of tile methods for a student to grasp fully the principles that underlie what he is doing, but references to detailed treatments are given. There is a particularly useful section (complete with protractors) on correcting ultraviolet absorbance readings for light scatter, and a description of a new variant of the microprecipitin serological test.

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