Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer (Volume 207) (Methods in Cell Biology, Volume 207)

Advanced Methodologies to Study and Enhance Cellular Cytotoxicity Against Cancer, Volume 207 covers the latest techniques and strategies used to investigate and boost cytotoxic activity in cells targeting cancer. Chapters include An ex vivo assay to assess CAR T cell cytotoxicity in viable tumor tissues using a two-step staining protocol, New protocol to measure mitochondria polarization/function at the immunological synapse, Analysis of CAR-T cell–mediated cytotoxicity in solid tumor models, Investigation of the in vitro lytic capacity of CAR-T by microscopyal, Antigen-specific T cell cytotoxicity toward target tumor cells, In vitro expansion of stem-like memory T cells via D-mannose supplementation, and much more.

Additional chapters explore High-throughput time-lapse imaging of B16 target cell killing by ovalbumin-specific CD8+ T cells, Evaluation of an intracellular magneto-mechanical approach for regulating dendritic cell activation, Analyzing the effect of NK cells on in-vivo models of tumor dissemination by digital pathology and flow cytometry, Super-resolution imaging of the NK cell synapse on supported lipid bilayers using MINFLUX nanoscopy, ASSESSMENT OF NK CELL CYTOTOXICITY INDUCED BY IL-15 BASED IMMUNOTHERAPY AGAINST CANCER CELLS, Combined phenotyping and functional analysis of isolated human natural killer cells using spectral flow cytometry, and much more.

• Presents state-of-the-art methodologies for investigating cellular cytotoxicity in cancer research
• Highlights advanced experimental and analytical approaches, including flow cytometry, live-cell imaging, and molecular profiling
• Explores strategies to enhance the cytotoxic potential of immune and therapeutic cells against various cancer types

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.

Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:
OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience.

Clément Thomas, Ph.D. is a research group leader at the Department of Cancer Research of the Luxembourg Institute of Health, Luxembourg. He received his Ph.D. in Molecular and Cellular Biology from the University of Strasbourg. Over the last 15 years, his group has developed extensive knowledge and expertise in the mechanisms regulating actin cytoskeleton organization and dynamics, as well as in live cell imaging approaches. His current research is centered on the role of the actin cytoskeleton in tumor immune evasion.