Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual

Advanced Methods in Molecular Biology and Biotechnology: A Practical Lab Manual is a concise reference on common protocols and techniques for advanced molecular biology and biotechnology experimentation. Each chapter focuses on a different method, providing an overview before delving deeper into the procedure in a step-by-step approach. Techniques covered include genomic DNA extraction using cetyl trimethylammonium bromide (CTAB) and chloroform extraction, chromatographic techniques, ELISA, hybridization, gel electrophoresis, dot blot analysis and methods for studying polymerase chain reactions. Laboratory protocols and standard operating procedures for key equipment are also discussed, providing an instructive overview for lab work.

This practical guide focuses on the latest advances and innovations in methods for molecular biology and biotechnology investigation, helping researchers and practitioners enhance and advance their own methodologies and take their work to the next level.

• Explores a wide range of advanced methods that can be applied by researchers in molecular biology and biotechnology
• Features clear, step-by-step instruction for applying the techniques covered
• Offers an introduction to laboratory protocols and recommendations for best practice when conducting experimental work, including standard operating procedures for key equipment

Khalid Z. Masoodi is an Associate Professor and Senior Scientist in the Division of Plant Biotechnology at Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, India. He is the recipient of a number of awards and holds numerous patents. He has two decades worth of teaching and research experience in the areas of plant biotechnology, molecular biology, cancer research, and human disease research, and has published numerous articles and books. He leads a transcriptomics laboratory and works extensively with fluorescence imaging, real-time PCR, cell culture, and Western blotting platforms. His scientific interests include transcription biology in plant stress responses and cancer, as well as the discovery of new drugs from medicinal plants.

Sameena Maqbool Lone is a biotechnology researcher with a strong focus on vegetable science, molecular biotechnology, and sustainable bioproduction. She is currently a postdoctoral researcher in the Division of Vegetable Science, Sher-e- Kashmir University of Agricultural Sciences and Technology of Kashmir, India. Her current work explores the modulation of inflammatory and oncogenic signalling pathways, positioning plant-derived compounds within the broader framework of translational cancer therapeutics. Her research interests include phytochemical science, molecular diversity analysis, and the application of plant resources for health-related innovation. She holds a number of patents and has co-authored several research papers and books.

Rovidha Saba Rasool did her Bachelors in Agriculture from SKUAST-K, Wadura followed by Masters in Plant Pathology from SKUAST-Kashmir, Shalimar. Currently, she is pursuing her Ph.D from Division of Plant Pathology, SKUAST-Kashmir. She has done commendable work in finding new genes involved in the pathogenesis of shot hole in stone fruits through insertional mutagenesis in Thyrostroma carpophilum. She has been a good student holding an excellent academic and extracurricular record and was awarded Ph.D. merit scholarship by SKUAST-K, in 2018-19. Apart from this, Rovidha has taken part in various national-level sports competitions and has won many national and state-level awards in baseball and handball.