Advances in Different Methods for The Detection of Contagious Diseases

Different isothermal amplification techniques are the signal amplification assays, the probe amplification assays, and the target amplification assays. Molecular techniques used for detection of quinolone resistance consist of the use of various techniques like polymerase chain reaction fragment length polymorphism, PCR, single-strand conformation polymorphism, nucleotide-sequencing analysis and multiplex allele-specific polymerase chain reaction. Main nucleic acid testing techniques are amplified nucleic acid techniques, microarrays, and non-amplified acid techniques. Recombinase aided amplification (RAA) assay has been successfully applied in the detection of bacterial and viral pathogens and overcomes the technical difficulties posed by DNA amplification methods because it does not need thermal denaturation of the template and operates at a low and constant temperature.