Advances in Single Cell Analysis: From Fundamentals to Translational Research

The book explores single-cell analysis, enabling the study of individual cells within a population. It presents recent studies on genomics, transcriptomics, proteomics, metabolomics, and other cellular aspects at the single-cell level.

Single-cell analysis primarily involves examining or manipulating individual cells under specific conditions using optical or electronic microscopes, time-lapse imaging, or confocal microscopy. It is widely used for high-throughput drug screening and evaluating drug effects. Importantly, single-cell analysis allows researchers to uncover cellular heterogeneity within populations, a crucial factor in various scientific and medical fields, including biology, medicine, and biotechnology.

This book provides a comprehensive overview of single-cell analysis, covering fundamental concepts, emerging technologies, and applications across single-cell omics, single-cell RNA and DNA sequencing, and image processing. It highlights the applications of single-cell analysis in synthetic gene networks, bacterial infections, stem cell research, induced pluripotent stem cells (iPSCs), cancer, cardiovascular diseases, neurological disorders, precision medicine, and regenerative medicine.

By addressing a wide range of topics, this book fills a critical gap in literature, making it an invaluable resource for PhD students, senior researchers and faculty members in molecular and cellular biology, biomedical sciences, and biotechnology. Considering the relevance of single-cell analysis in industrial biotechnology, the book is also aimed at industry professionals in diagnostics, pharmaceuticals, and personalized medicine.

Dr. Vijai Singh is a full Professor and Head of the Department of Biosciences, School of Science at Indrashil University, Rajpur, Mehsana, India. He has worked as Associate Professor in the Department of Biosciences, School of Science at Indrashil University, Rajpur, Mehsana, India. He was an Assistant Professor in the Department of Biological Sciences and Biotechnology at the Institute of Advanced Research, Gandhinagar, India and also an Assistant Professor in the Department of Biotechnology at the Invertis University, Bareilly, India. Prior to that, he was a Postdoctoral Fellow in the Synthetic Biology Group at the Institute of Systems and Synthetic Biology, Paris, France and School of Energy & Chemical Engineering at the Ulsan National Institute of Science and Technology, Ulsan, South Korea. He received his Ph.D. in Biotechnology (2009) from the National Bureau of Fish Genetic Resources, Uttar Pradesh Technical University, Lucknow, India with a research focus on the development of molecular and immunoassays for diagnosis of Aeromonas hydrophila. His research interests are focused on building novel biosynthetic pathways for production of medically and industrially important biomolecules. Additionally, his laboratory is working on CRISPR-Cas9 tools for genome editing. He has more than 12 years of research and teaching experience in synthetic biology, metabolic engineering, bioinformatics, microbiology, and industrial microbiology. He has published 110 articles, 80 book chapters, 27 books and 3 patents. He serves as an associate editor, editorial board member, and reviewer of several peer-reviewed journals. He is also a member of the Board of Study and Academic Council of Indrashil University and is the Member Secretary of the Institutional Biosafety Committee (IBSC) at the same University.