Forty years after the term “affinity chromatography” was introduced, this mode of chromatography remains a key tool in the armory of separation techniques that are available to separation and interaction scientists. Affinity chromat- raphy is favored because of its high selectivity, speed, and ease of use. The rapid and selective isolation of molecules using affinity chromatography has allowed a better understanding of biological processes, accelerated the identi- cation of target molecules, and spawned new process areas such as immobilized enzyme reactors. It has had ubiquitous application in most areas of science ranging from small molecule isolation to biopolymers such as DNA, proteins, polysaccharides, and even whole cells. The number of applications of affinity chromatography continues to expand at a rapid rate. For example, more than 60% of purification protocols include some sort of affinity chromatography step, while a database search of PubMed reveals more than 36,000 publications making use of the term “affinity chromatography,” more than 3000 of which refer to it in their title. The US patent office reports more than 16,000 ref- ences to the term “affinity chromatography”, while there are more than 270 references to the same term in the patent title. The aim of this edition of Methods in Molecular Biology, Affinity Chromatography: Methods and Protocols, Second Edition is to provide the beginner with the practical knowledge to develop affinity separations suitable for various applications relevant to the post-genomic era.
Thirty-eight years after its introduction, affinity chromatography remains a key tool in the armory of separation techniques available to separation and interaction scientists. Expanded and updated from the first edition, Affinity Chromatography: Methods and Protocols, Second Edition, provides the beginner with the practical knowledge needed to develop affinity separations suitable for a variety of applications relevant to the post-genomic era. This second edition expands on the first edition by introducing more state-of-the-art protocols used in affinity chromatography. This new edition also describes protocols that demonstrate the concept of affinity chromatography being applied to meet the modern high throughput screening demands of researchers and development scientists whilst expanding on some more traditional affinity chromatography approaches that have become of greater interest to separation scientists. Chapters in this cutting-edge text expand on affinity chromatography techniques that currently enjoy frequent citation in the literature from those purifying biomolecules. Other chapters include protocols describing the use of a variety of fusion tags as well as how to cleave them, so as to allow the scientists to study the native phenotype of the protein. Renowned researchers also include protocols detailing diverse applications of affinity chromatography such as its use in catalytic reactions, DNA purification, whole cell separations and for the isolation of phosphorylated proteins. Affinity Chromatography: Methods and Protocols, Second Edition, is an essential reference for those interested in separation sciences, particularly in the pharmaceutical and biological research sectors, that have an interest in isolating macromolecules rapidly, quantitatively, and with high purity.
