Agrobacterium Protocols: Volume II (Volume 344)

. Ed(s): Kan Wang

ISBN 10: 1588298434 / ISBN 13: 9781588298430

Published by Humana Press, 2006

About this Item

A collection of Agrobacterium-mediated transformation techniques for advanced plant genetic engineering, functional genomic analysis, and crop improvement. It details the techniques available for twenty-six plant species drawn from cereal crops, industrial plants, legume plants, and vegetable plants. Editor(s): Kan Wang. Series: Methods in Molecular Biology. Num Pages: 512 pages, 81 black & white illustrations, 6 colour illustrations, biography. BIC Classification: PSD; PSF; PST. Category: (P) Professional & Vocational. Dimension: 235 x 155 x 33. Weight in Grams: 953. . 2006. 2nd Edition. hardcover. . . . . Books ship from the US and Ireland.

Seller Inventory # V9781588298430

Bibliographic Details

• Title: Agrobacterium Protocols: Volume II (Volume 344)
• Publisher: Humana Press
• Publication Date: 2006
• Language: English
• ISBN 10: 1588298434
• ISBN 13: 9781588298430
• Binding: Hardcover
• Edition: 2nd Edition
• Condition: New

About this title

Synopsis

Agrobacterium tumefaciens is a soil bacterium that for more than a century has been known as a pathogen causing the plant crown gall disease. Unlike many other pathogens, Agrobacterium has the ability to deliver DNA to plant cells and permanently alter the plant genome. The discovery of this unique feature 30 years ago has provided plant scientists with a powerful tool to genetically transform plants for both basic research purposes and for agric- tural development. Compared to physical transformation methods such as particle bomba- ment or electroporation, Agrobacterium-mediated DNA delivery has a number of advantages. One of the features is its propensity to generate single or a low copy number of integrated transgenes with defined ends. Integration of a single transgene copy into the plant genome is less likely to trigger “gene silencing” often associated with multiple gene insertions. When the first edition of Agrobacterium Protocols was published in 1995, only a handful of plants could be routinely transformed using Agrobacterium. Ag- bacterium-mediated transformation is now commonly used to introduce DNA into many plant species, including monocotyledon crop species that were previously considered non-hosts for Agrobacterium. Most remarkable are recent devel- ments indicating that Agrobacterium can also be used to deliver DNA to non-plant species including bacteria, fungi, and even mammalian cells.

Review

From the reviews of the second edition:

"This is, as the title suggests, a laboratory manual with protocols for using Agrobacterium tumefaciens to transform plant species ... . the book will be a valuable addition to the laboratory bookshelf. The protocols ... are clearly set out and the notes explain things ... . I was also pleased that some chapters mentioned the need to consider regulatory issues regarding disposal of genetically modified materials, something that is often ignored in lab manuals." (Heather Macdonald, SGM – Society for General Microbiology, 2007)