Allosteric Interactions in P2X Receptor Subunits: Photolabeling and Fluorescence Technologies to study P2X receptors

Bhargava, Yogesh

ISBN 10: 6202025417 / ISBN 13: 9786202025416

Published by LAP LAMBERT Academic Publishing, 2017

About this Item

200 pages. 8.66x5.91x0.46 inches. In Stock.

Seller Inventory # zk6202025417

Bibliographic Details

• Title: Allosteric Interactions in P2X Receptor Subunits: Photolabeling and Fluorescence Technologies to study P2X receptors
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2017
• Language: English
• ISBN 10: 6202025417
• ISBN 13: 9786202025416
• Binding: Paperback
• Condition: Brand New
• Weight: 0.35 kilograms

About this title

Synopsis

P2X receptors represent the third superfamily of ligand gated ion channels with ATP as their natural ligand. In spite of them being an attractive drug target, their potential as a drug target is limited by the lack of basic understanding of the structure-function relationship of these receptors. In this work, I have investigated the behaviour of homomeric P2X receptor subunits with the help of photolabeling and fluorescence techniques coupled with electrophysiological measurements using Xenopus laevis oocytes heterologous expression system. Photolabeling system offered real time labelling of P2X receptors and concurrent measurement of current from the same set of receptors. Also, this study for the first time describes the agonist potency of Alexa-ATP (a fluorescent ATP analogue) on P2X1 receptors. Using these technologies, it was found that the receptor subunits are not independent but interacting with each other in a cooperative manner. This work has attempted to answer few intriguing questions in the field of P2X receptor research and I think that the answers provide many avenues to the basic understanding of the functioning of P2X receptors.

About the Author

Dr. Yogesh Bhargava is an Assistant Professor at Dr. Harisingh Gour Central University, Sagar, MP, India. He did his PhD from Max Planck Institute of Biophysics, Frankfurt, Germany. He is an expert of Zebrafish Behavioural Neuroscience and P2X receptors and interested in Technology development.