Analytical Method Development and Validation

Language: English

Published by LAP LAMBERT Academic Publishing Sep 2012, 2012

3659253995 / 9783659253997

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This item is printed on demand - Print on Demand Titel. Neuware -A simple reverse phase liquid Chromatographic method has been developed and subsequently validated for simultaneous determination of Pioglitazone and Glimepiride in combination. The separation was carried out using a mobile phase of phosphate buffer (pH-4.5): Acetonitrile (45:55) v/v and using methanol as diluent. The column used was Inertsil ODS (250 mm x 4.6 mm i.d., 5¿m) with flow rate of 1 ml/min using UV detection at 225 nm. The described method was linear over a concentration range of 5-50¿g/ml and 5-25 ¿g/ml for the assay of Pioglitazone and Glimepiride respectively. The retention times of Pioglitazone and Glimepiride were found to be4.6 and 7.7min respectively. Results of analysis were validated statistically and by recovery studies. The results of the study showed that the proposed RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of Pioglitazone and Glimepiride bulk drug and in its pharmaceutical dosage form.VDM Verlag, Dudweiler Landstraße 99, 66123 Saarbrücken 112 pp. Englisch.…

Seller Inventory # 9783659253997

Title
Analytical Method Development and Validation
Author
Gagan Sharma
Publisher
LAP LAMBERT Academic Publishing Sep 2012
Publication year
2012
Condition
Neu
Dust jacket
Dust Jacket Included
Binding
Taschenbuch
Language
English
ISBN 10
3659253995
ISBN 13
9783659253997
Item weight
185 grams
Dimensions
220x150x8 mm

buchversandmimpf2000

Emtmannsberg, BAYE, Germany

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