Animal Cell and Tissue Culture

Contents 1. Cells in culture an introduction. 2. Tissue culture medium. 3. Initiation of cell culture primary culture. 4. Cell lines. 5. Selection and cloning of cells. 6. Characterization and preservation of animal cells. 7. Scale up of animal cell culture. 8. Fermentors. 9. Hybridoma Technology and monoclonal antibodies. 10. Genetic manipulation of cells transgenesis. 11. Applications of transgenic animals. 12. Tissue grafting and engineering. 13. Gene therapy. References. Subject index. Man is considered to be the most powerful creature in universe but he depends totally on the biological entities for his all nutritional needs. Biotechnology use biological entities for the betterment of human kind. Biotechnology derived from fusion of biology and technology is a very vast subject which includes plant cell and tissue culture animal cell and tissue culture transgenic molecular biology recombinant DNA technology genetic engineering medical biotechnology fermentation technology microbiology enzyme technology biofuels environmental biotechnology bioinformatics and nanotechnolgy. It may be pointed out that use of biological entities is not new. Ever since establishment of civilization man is using biological organisms to satisfy his needs. But this can not be regarded as biotechnology since production technology pertaining to agriculture and animal husbandry etc. has now become distinct disciplines. However exploitation of plant and animal cell in vitro as well as their constituents to generate some useful product constitute biotechnology. Animal cell and tissue culture is an integral part of biotechnology and this book covers all the aspects of animal cell culture. Animal cells are used for making new vaccines specific animal proteins such as intergerons blood factors and hormones monoclonal antibodies for use as diagnostic and therapeutics gene probes as diagnostic too enzymes and last but not the least many new and important compounds. This book is beneficial to all students of under graduation as well as post graduation of all universities. 266 pp.…