Anther Culture and Somatic Embryogenesis in Rice: Production of Haploid Plants and Somatic Embryo Induction & Plant Regeneration from Different Explants in Rice

Shahnewaz, Sharmin, Islam, Rafiul, Hossain, Monzur

ISBN 10: 3847327631 / ISBN 13: 9783847327639

Published by LAP LAMBERT Academic Publishing, 2012

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Bibliographic Details

• Title: Anther Culture and Somatic Embryogenesis in Rice: Production of Haploid Plants and Somatic Embryo Induction & Plant Regeneration from Different Explants in Rice
• Publisher: LAP LAMBERT Academic Publishing
• Publication Date: 2012
• Language: English
• ISBN 10: 3847327631
• ISBN 13: 9783847327639
• Binding: paperback
• Condition: New
• Book Type: book

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Synopsis

The first part of this investigation deals with induction of callus and regeneration of haploid plants from anther cultures of rice. Z2 containing 2,4-D 0.5 mg/l+NAA 2.5 mg/l+KIN 0.5 mg/l induced highest 6.36% callus in BRRI dhan-29. Medium containing 4.0% sucrose gave the highest 7.5% callus formation as well as highest green plants regeneration. Pretreatment of cold shock is beneficial for callus induction and regeneration of haploid plants. Chromosomes counting was made from root tips and observed 69.0% haploids (n=12) in BRRI dhan-29. In the second part effect of three basal media MS, N6 and LS supplemented with four growth regulator treatments on the response of induction of callus from three different sources of explants like mature seed scutella, roots and immature embryos of four rice genotypes was studied. The highest callus formation 88.8% was recorded in Taipei-309 from mature seed scutella. Calli were subcultured with tryptophan in MS+2,4-D(1.0 & 1.5 mg/l)+NAA 0.5 mg/l for somatic embryogenesis. Regeneration response of embryogenic calli was tested on RM1, RM2 and RM3 and highest 79.5% calli formed shoots in BRRI dhan-29 from immature embryos in RM3.

About the Author

Sharmin Shahnewaj, MSc, PhD: Studied Botany at Rajshahi Univ, Rajshahi, Bangladesh.Rafiul Islam, MPhil, PhD: Studied Botany at Rajshahi Univ, Rajshahi, Bangladesh. Prof of Botany, Rajshahi Univ.Monzur Hossain, PhD (Bangladesh), PhD (Japan): Studied Botany at Rajshahi Univ, Rajshahi, Bangladesh. Prof of Botany, Rajshahi Univ.